Anti-NEFM Antibody (CAB16405)
- SKU:
- CAB16405
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NEFM Antibody |
| 抗体コード: | CAB16405 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NEFM (NP_005373.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | SH-SY5Y |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NEFM (NP_005373.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EAAI RALR KDIE EASL VKVE LDKK VQSL QDEV AFLR SNHE EEVA DLLA QIQA SHIT VERK DYLK TDIS TALK EIRS QLES HSDQ NMHQ AEEW FKCR YAKL T |
| 遺伝子ID: | 4741 |
| Uniprot: | P07197 |
| セルラーロケーション: | |
| 計算された分子量: | 59kDa/102kDa |
| 観察された分子量: | 160KDa |
| 同義語: | NEF3, NF-M, NFM, NEFM |
| バックグラウンド: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 8p21 Cellular Component: axon; neurofilament; neuromuscular junction Molecular Function:protein binding; structural constituent of cytoskeleton; microtubule binding Biological Process: neurofilament bundle assembly; axon cargo transport; regulation of axon diameter; microtubule cytoskeleton organization and biogenesis |
| NCBI Summary: | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| UniProt Code: | P07197 |
| NCBI GenInfo Identifier: | 281185500 |
| NCBI Gene ID: | 4741 |
| NCBI Accession: | P07197.3 |
| UniProt Secondary Accession: | P07197,Q4QRK6, B4DGN2, E9PBF7, |
| UniProt Related Accession: | P07197 |
| Molecular Weight: | 59,599 Da |
| NCBI Full Name: | Neurofilament medium polypeptide |
| NCBI Synonym Full Names: | neurofilament, medium polypeptide |
| NCBI Official Symbol: | NEFM |
| NCBI Official Synonym Symbols: | NFM; NEF3; NF-M |
| NCBI Protein Information: | neurofilament medium polypeptide; neurofilament 3; 160 kDa neurofilament protein; neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide 150kDa |
| UniProt Protein Name: | Neurofilament medium polypeptide |
| UniProt Synonym Protein Names: | 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein |
| Protein Family: | Neurofilament medium polypeptide |
| UniProt Gene Name: | NEFM |
| UniProt Entry Name: | NFM_HUMAN |
 | Western blot analysis of extracts of SH-SY5Y cells, using NEFM Rabbit pAb (CAB16405) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using NEFM Rabbit pAb (CAB16405) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using NEFM Rabbit pAb (CAB16405) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of mouse brain using NEFM antibody (CAB16405) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of rat brain using NEFM antibody (CAB16405) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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