Anti-NEDD1 Antibody (CAB5128)
- SKU:
- CAB5128
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-NEDD1 Antibody |
| 抗体コード: | CAB5128 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human NEDD1 (NP_690869.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, 293T, Jurkat, mouse brain, rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human NEDD1 (NP_690869.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | SPIR DDAV VNKG SDES IGKG DGFD FLPQ LNSV FPPR KNPV TSST SVLH SSPL NVFM GSPG KEEN ENRD LTAE SKKI YMGK QESK DSFK QLAK LVTS GAES GNLN TSPS SNQT RNSE KFEK PENE IEAQ LICE PPIN GSST PNPK IASS VTAG VASS LSEK IADS IGNN RQNA PLTS IQIR FIQN MIQE TLDD FREA CHRD IVNL QVEM IKQF HMQL NEMH SLLE RYSV NEGL VAEI ERLR EENK RLRA HF |
| 遺伝子ID: | 121441 |
| Uniprot: | Q8NHV4 |
| セルラーロケーション: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 62kDa/71kDa/72kDa |
| 観察された分子量: | 78kDa |
| 同義語: | NEDD1, GCP-WD, TUBGCP7 |
| バックグラウンド: |
| UniProt Protein Function: | NEDD1: Required for mitosis progression. Promotes the nucleation of microtubules from the spindle. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 12q23.1 Cellular Component: centriole; nucleoplasm; spindle pole; centrosome; pericentriolar material; apical part of cell; nucleolus; plasma membrane; cytosol Biological Process: mitosis; cell division; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle |
| UniProt Code: | Q8NHV4 |
| NCBI GenInfo Identifier: | 74762597 |
| NCBI Gene ID: | 121441 |
| NCBI Accession: | Q8NHV4.1 |
| UniProt Secondary Accession: | Q8NHV4,Q8NA30, B0AZN0, B4E145, G3V3F1, |
| UniProt Related Accession: | Q8NHV4 |
| Molecular Weight: | 660 |
| NCBI Full Name: | Protein NEDD1 |
| NCBI Synonym Full Names: | neural precursor cell expressed, developmentally down-regulated 1 |
| NCBI Official Symbol: | NEDD1 |
| NCBI Official Synonym Symbols: | GCP-WD; TUBGCP7 |
| NCBI Protein Information: | protein NEDD1; NEDD-1; neural precursor cell expressed developmentally down-regulated protein 1 |
| UniProt Protein Name: | Protein NEDD1 |
| UniProt Synonym Protein Names: | Neural precursor cell expressed developmentally down-regulated protein 1; NEDD-1 |
| Protein Family: | Protein |
| UniProt Gene Name: | NEDD1 |
| UniProt Entry Name: | NEDD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NEDD1 antibody (CAB5128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using NEDD1 antibody (CAB5128) at dilution of 1:100. Blue: DAPI for nuclear staining. |