Anti-NDUFS8 Antibody (CAB13034)
- SKU:
- CAB13034
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NDUFS8 Antibody |
| 抗体コード: | CAB13034 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-210 of human NDUFS8 (NP_002487.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Mouse heart, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-210 of human NDUFS8 (NP_002487.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | TYKY VNMQ DPEM DMKS VTDR AART LLWT ELFR GLGM TLSY LFRE PATI NYPF EKGP LSPR FRGE HALR RYPS GEER CIAC KLCE AICP AQAI TIEA EPRA DGSR RTTR YDID MTKC IYCG FCQE ACPV DAIV EGPN FEFS TETH EELL YNKE KLLN NGDK WEAE IAAN IQAD YLYR |
| 遺伝子ID: | 4728 |
| Uniprot: | O00217 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 23kDa |
| 観察された分子量: | 23kDa |
| 同義語: | NDUFS8, CI-23k, CI23KD, TYKY |
| バックグラウンド: | This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. |
| UniProt Protein Function: | TYKY: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone. Defects in NDUFS8 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Belongs to the complex I 23 kDa subunit family. |
| UniProt Protein Details: | Protein type:EC 1.6.99.3; EC 1.6.5.3; Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase Chromosomal Location of Human Ortholog: 11q13 Cellular Component: mitochondrial matrix; mitochondrial respiratory chain complex I; mitochondrion Molecular Function:NADH dehydrogenase (ubiquinone) activity; NADH dehydrogenase activity Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; response to oxidative stress Disease: Leigh Syndrome |
| NCBI Summary: | This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010] |
| UniProt Code: | O00217 |
| NCBI GenInfo Identifier: | 2499325 |
| NCBI Gene ID: | 4728 |
| NCBI Accession: | O00217.1 |
| UniProt Secondary Accession: | O00217,Q0VDA8, B2RB86, |
| UniProt Related Accession: | O00217 |
| Molecular Weight: | 23,705 Da |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase core subunit S8 |
| NCBI Official Symbol: | NDUFS8 |
| NCBI Official Synonym Symbols: | TYKY; CI-23k; CI23KD |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial |
| UniProt Synonym Protein Names: | Complex I-23kD; CI-23kD; NADH-ubiquinone oxidoreductase 23 kDa subunit; TYKY subunit |
| UniProt Gene Name: | NDUFS8 |
| UniProt Entry Name: | NDUS8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFS8 antibody (CAB13034) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using NDUFS8 Polyclonal Antibody (CAB13034) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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