Anti-NDUFS2 Antibody (CAB12858)[KO Validated]
![Anti-NDUFS2 Antibody (CAB12858)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/25352/25200/anti-ndufs2-antibody-cab12858ko-validated__23000__40043.1645806246.jpg?c=1 "Anti-NDUFS2 Antibody (CAB12858)[KO Validated]")
- SKU:
- CAB12858
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
![Anti-NDUFS2 Antibody (CAB12858)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/25352/25200/anti-ndufs2-antibody-cab12858ko-validated__23000__40043.1645806246.jpg?c=1&imbypass=on "Anti-NDUFS2 Antibody (CAB12858)[KO Validated]")
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Description
| 抗体名: | Anti-NDUFS2 Antibody [KO Validated] |
| 抗体コード: | CAB12858 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human NDUFS2 (NP_004541.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LO2, HeLa, 293T, Mouse brain, Mouse kidney, Mouse spleen, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human NDUFS2 (NP_004541.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FEER EKMF EFYE RVSG ARMH AAYI RPGG VHQD LPLG LMDD IYQF SKNF SLRL DELE ELLT NNRI WRNR TIDI GVVT AEEA LNYG FSGV MLRG SGIQ WDLR KTQP YDVY DQVE FDVP VGSR GDCY DRYL CRVE EMRQ SLRI IAQC LNKM PPGE IKVD DAKV SPPK RAEM KTSM ESLI HHFK LYTE GYQV PPGA TYTA IEAP KGEF GVYL VSDG SSRP YRCK IKAP GFAH LAGL DKMS KGHM LADV VAII GTQD IVFG EVDR |
| 遺伝子ID: | 4720 |
| Uniprot: | O75306 |
| セルラーロケーション: | Matrix side, Mitochondrion inner membrane, Peripheral membrane protein |
| 計算された分子量: | 51kDa/52kDa |
| 観察された分子量: | 49kDa |
| 同義語: | NDUFS2, CI-49 |
| バックグラウンド: | The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | NDUFS2: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Defects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I 49 kDa subunit family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Mitochondrial; EC 1.6.5.3; EC 1.6.99.3; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 1q23 Cellular Component: mitochondrial matrix; mitochondrial respiratory chain complex I; mitochondrion; nucleoplasm Molecular Function:NADH dehydrogenase activity; protein binding; ubiquitin protein ligase binding Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; response to oxidative stress Disease: Mitochondrial Complex I Deficiency |
| NCBI Summary: | The protein encoded by this gene is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mutations in this gene are associated with mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009] |
| UniProt Code: | O75306 |
| NCBI GenInfo Identifier: | 20178314 |
| NCBI Gene ID: | 4720 |
| NCBI Accession: | O75306.2 |
| UniProt Secondary Accession: | O75306,Q5VTW0, Q969P3, Q9UEV3, D3DVG7, J3KPM7, |
| UniProt Related Accession: | O75306 |
| Molecular Weight: | 51,852 Da |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase core subunit S2 |
| NCBI Official Symbol: | NDUFS2 |
| NCBI Official Synonym Symbols: | CI-49 |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial |
| UniProt Synonym Protein Names: | Complex I-49kD; CI-49kD; NADH-ubiquinone oxidoreductase 49 kDa subunit |
| UniProt Gene Name: | NDUFS2 |
| UniProt Entry Name: | NDUS2_HUMAN |
![Anti-NDUFS2 Antibody [KO Validated] (CAB12858)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/j/636/A12858_1__02493.jpg) | Western blot analysis of extracts of various cell lines, using NDUFS2 antibody (CAB12858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
![Anti-NDUFS2 Antibody [KO Validated] (CAB12858)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/w/407/A12858_2__36654.jpg) | Western blot analysis of extracts from normal (control) and NDUFS2 knockout (KO) 293T cells, using NDUFS2 antibody (CAB12858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
![Anti-NDUFS2 Antibody [KO Validated] (CAB12858)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/s/099/A12858_3__14023.jpg) | Immunofluorescence analysis of L929 cells using NDUFS2 antibody (CAB12858) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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