Anti-NDUFB5 Antibody (CAB8327)
- SKU:
- CAB8327
- Product type:
- Antibody
- Application:
- WB
- Application:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NDUFB5 Antibody |
| 抗体コード: | CAB8327 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, Mouse kidney, Rat kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-189 of human NDUFB5 (NP_002483.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EIPE GYVP EHWE YYKH PISR WIAR NFYD SPEK IYER TMAV LQIE AEKA ELRV KELE VRKL MHVR GDGP WYYY ETID KELI DHSP KATP DN |
| 遺伝子ID: | 4711 |
| Uniprot: | O43674 |
| セルラーロケーション: | Matrix side, Mitochondrion inner membrane, Single-pass membrane protein |
| 計算された分子量: | 15kDa/21kDa |
| 観察された分子量: | 22kDa |
| 同義語: | NDUFB5, CISGDH, SGDH |
| バックグラウンド: | The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| NCBI Summary: | The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011] |
| UniProt Code: | O43674 |
| NCBI GenInfo Identifier: | 316660086 |
| NCBI Gene ID: | 4711 |
| NCBI Accession: | NP_001186886.1 |
| UniProt Secondary Accession: | O43674,Q561V6, |
| UniProt Related Accession: | O43674 |
| Molecular Weight: | 20 |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase subunit B5 |
| NCBI Official Symbol: | NDUFB5 |
| NCBI Official Synonym Symbols: | SGDH; CISGDH |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial |
| UniProt Synonym Protein Names: | Complex I-SGDH; CI-SGDH; NADH-ubiquinone oxidoreductase SGDH subunit |
| UniProt Gene Name: | NDUFB5 |
 | Western blot analysis of extracts of various cell lines, using NDUFB5 antibody (CAB8327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s. |
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