Anti-NDUFB5 Antibody (CAB19953)[KO Validated]

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SKU:
CAB19953
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
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Description

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抗体名:Anti-NDUFB5 Antibody [KO Validated]
抗体コード:CAB19953
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:Recombinant protein of human NDUFB5.
申し込み:WB IHC
推奨希釈:WB 1:500 - 1:2000 IHC 1:50 - 1:100
反応性:Human, Mouse, Rat
ポジティブサンプル:HeLa
免疫原:Recombinant protein of human NDUFB5.
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:Email for sequence
遺伝子ID:4711
Uniprot:O43674
セルラーロケーション:Matrix side, Mitochondrion inner membrane, Single-pass membrane protein
計算された分子量:15kDa/21kDa
観察された分子量:16KDa
Anti-NDUFB5 Antibody [KO Validated] (CAB19953)Western blot analysis of extracts from normal (control) and NDUFB5 knockout (KO) HeLa cells, using NDUFB5 antibody (CAB19953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
同義語:NDUFB5, CISGDH, SGDH
バックグラウンド:The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
NCBI Summary:The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:O43674
NCBI GenInfo Identifier:316660086
NCBI Gene ID:4711
NCBI Accession:NP_001186886.1
UniProt Secondary Accession:O43674,Q561V6,
UniProt Related Accession:O43674
Molecular Weight:20
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH:ubiquinone oxidoreductase subunit B5
NCBI Official Symbol:NDUFB5  
NCBI Official Synonym Symbols:SGDH; CISGDH  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
UniProt Synonym Protein Names:Complex I-SGDH; CI-SGDH; NADH-ubiquinone oxidoreductase SGDH subunit
UniProt Gene Name:NDUFB5  
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