Anti-NDUFB3 Antibody (CAB14378)
- SKU:
- CAB14378
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NDUFB3 Antibody |
| 抗体コード: | CAB14378 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human NDUFB3 (NP_002482.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | Mouse heart, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human NDUFB3 (NP_002482.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAHE HGHE HGHH KMEL PDYR QWKI EGTP LETI QKKL AAKG LRDP WGRN EAWR YMGG FAKS VSFS DVFF KGFK WGFA AFVV AVGA EYYL ESLN KDKK HH |
| 遺伝子ID: | 4709 |
| Uniprot: | O43676 |
| セルラーロケーション: | Matrix side, Mitochondrion inner membrane, Single-pass membrane protein |
| 計算された分子量: | 11kDa |
| 観察された分子量: | 11kDa |
| 同義語: | NDUFB3, B12, CI-B12 |
| バックグラウンド: | This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene. |
| UniProt Protein Function: | NDUFB3: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB3 subunit family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; EC 1.6.99.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial; EC 1.6.5.3; Oxidoreductase Chromosomal Location of Human Ortholog: 2q31.3 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency |
| NCBI Summary: | This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene. [provided by RefSeq, Mar 2012] |
| UniProt Code: | O43676 |
| NCBI GenInfo Identifier: | 6093472 |
| NCBI Gene ID: | 4709 |
| NCBI Accession: | O43676.3 |
| UniProt Secondary Accession: | O43676,Q6IB80, |
| UniProt Related Accession: | O43676 |
| Molecular Weight: | 11,402 Da |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase subunit B3 |
| NCBI Official Symbol: | NDUFB3 |
| NCBI Official Synonym Symbols: | B12; CI-B12 |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 |
| UniProt Synonym Protein Names: | Complex I-B12; CI-B12; NADH-ubiquinone oxidoreductase B12 subunit |
| Protein Family: | NADH dehydrogenase |
| UniProt Gene Name: | NDUFB3 |
| UniProt Entry Name: | NDUB3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFB3 antibody (CAB14378) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of L929 cells using NDUFB3 Polyclonal Antibody (CAB14378) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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