Anti-NDUFB2 Antibody (CAB3978)
- SKU:
- CAB3978
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NDUFB2 Antibody |
| 抗体コード: | CAB3978 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 34-105 of human NDUFB2 (NP_004537.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | NCI-H460, HeLa, 293T, Mouse brain, Mouse heart, Mouse kidney, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 34-105 of human NDUFB2 (NP_004537.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | AGGG VHIE PRYR QFPQ LTRS QVFQ SEFF SGLM WFWI LWRF WHDS EEVL GHFP YPDP SQWT DEEL GIPP DDED |
| 遺伝子ID: | 4708 |
| Uniprot: | O95178 |
| セルラーロケーション: | Matrix side, Mitochondrion inner membrane, Peripheral membrane protein |
| 計算された分子量: | 12kDa |
| 観察された分子量: | 12kDa |
| 同義語: | NDUFB2, AGGG, CI-AGGG |
| バックグラウンド: | The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I. |
| UniProt Protein Function: | NDUFB2: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB2 subunit family. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 7q34 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I Molecular Function:NADH dehydrogenase (ubiquinone) activity Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone |
| NCBI Summary: | The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays a important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Hydropathy analysis revealed that this subunit and 4 other subunits have an overall hydrophilic pattern, even though they are found within the hydrophobic protein (HP) fraction of complex I. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95178 |
| NCBI GenInfo Identifier: | 6093503 |
| NCBI Gene ID: | 4708 |
| NCBI Accession: | O95178.1 |
| UniProt Secondary Accession: | O95178,Q6FGI6, |
| UniProt Related Accession: | O95178 |
| Molecular Weight: | 105 |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa |
| NCBI Official Symbol: | NDUFB2 |
| NCBI Official Synonym Symbols: | AGGG; CI-AGGG |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial; complex I-AGGG; complex I AGGG subunit; NADH-ubiquinone oxidoreductase AGGG subunit |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial |
| UniProt Synonym Protein Names: | Complex I-AGGG; CI-AGGG; NADH-ubiquinone oxidoreductase AGGG subunit |
| UniProt Gene Name: | NDUFB2 |
| UniProt Entry Name: | NDUB2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFB2 antibody (CAB3978) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of L929 cells using NDUFB2 Polyclonal Antibody (CAB3978) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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