Anti-NDUFAF5 Antibody (CAB7135)
- SKU:
- CAB7135
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NDUFAF5 Antibody |
| 抗体コード: | CAB7135 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 196-345 of human NDUFAF5 (NP_077025.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, SGC7901 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 196-345 of human NDUFAF5 (NP_077025.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ELRC SLQL AETE REGG FSPH ISPF TAVN DLGH LLGR AGFN TLTV DTDE IQVN YPGM FELM EDLQ GMGE SNCA WNRK ALLH RDTM LAAA AVYR EMYR NEDG SVPA TYQI YYMI GWKY HESQ ARPA ERGS ATVS FGEL GKIN NLMP PGKK SQ |
| 遺伝子ID: | 79133 |
| Uniprot: | Q5TEU4 |
| セルラーロケーション: | Mitochondrion inner membrane |
| 計算された分子量: | 36kDa/38kDa |
| 観察された分子量: | 39kDa |
| 同義語: | NDUFAF5, C20orf7, bA526K24.2, dJ842G6.1 |
| バックグラウンド: | The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | NDUFAF5: Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I, MT-ND1) at early stages. May have methyltransferase activity. Defects in NDUFAF5 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Defects in NDUFAF5 are a cause of Leigh syndrome (LS). An early-onset progressive neurodegenerative disorder characterized by the presence of focal, bilateral lesions in one or more areas of the central nervous system including the brainstem, thalamus, basal ganglia, cerebellum and spinal cord. Clinical features depend on which areas of the central nervous system are involved and include subacute onset of psychomotor retardation, hypotonia, ataxia, weakness, vision loss, eye movement abnormalities, seizures, and dysphagia. Belongs to the methyltransferase superfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.1.1.-; Mitochondrial; Methyltransferase Chromosomal Location of Human Ortholog: 20p12.1 Molecular Function:methyltransferase activity Biological Process: methylation; mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency |
| NCBI Summary: | The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| UniProt Code: | Q5TEU4 |
| NCBI GenInfo Identifier: | 74762247 |
| NCBI Gene ID: | 79133 |
| NCBI Accession: | Q5TEU4.1 |
| UniProt Secondary Accession: | Q5TEU4,Q6GPH3, Q9H6F4, A8K166, |
| UniProt Related Accession: | Q5TEU4 |
| Molecular Weight: | 345 |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH dehydrogenase (ubiquinone) complex I, assembly factor 5 |
| NCBI Official Symbol: | NDUFAF5 |
| NCBI Official Synonym Symbols: | C20orf7; dJ842G6.1; bA526K24.2 |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5; probable methyltransferase C20orf7, mitochondrial |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 |
| UniProt Synonym Protein Names: | Probable methyltransferase C20orf7, mitochondrial (EC:2.1.1.-) |
| Protein Family: | Arginine-hydroxylase |
| UniProt Gene Name: | NDUFAF5 |
| UniProt Entry Name: | NDUF5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFAF5 antibody (CAB7135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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