Anti-NDUFAF4 抗体 (CAB14345)

抗体名:	Anti-NDUFAF4 Antibody
抗体コード:	CAB14345
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NDUFAF4 (NP_054884.1).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	U-87MG, HeLa, K-562

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NDUFAF4 (NP_054884.1).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MGAL VIRG IRNF NLEN RAER EISK MKPS VAPR HPST NSLL REQI SLYP EVKG EIAR KDEK LLSF LKDV YVDS KDPV SSLQ VKAA ETCQ EPKE FRLP KDHH FDMI NIKS IPKG KISI VEAL TLLN NHKL FPET WTAE KIMQ EYQL EQKD VNSL LKYF VTFE VEIF PPED KK
遺伝子ID:	29078
Uniprot:	Q9P032
セルラーロケーション:	Lipid-anchor, Membrane, Mitochondrion
計算された分子量:	20kDa
観察された分子量:	20kDa

同義語:	NDUFAF4, C6orf66, HRPAP20, HSPC125, My013, bA22L21.1
バックグラウンド:	NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.

UniProt Protein Function:	NDUFAF4: Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion. Defects in NDUFAF4 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the NDUFAF4 family.
UniProt Protein Details:	Protein type:Mitochondrial; Apoptosis Chromosomal Location of Human Ortholog: 6q16.1 Cellular Component: mitochondrial inner membrane; mitochondrial membrane; mitochondrion Molecular Function:protein binding Biological Process: mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency
NCBI Summary:	NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency. [provided by RefSeq, Oct 2009]
UniProt Code:	Q9P032
NCBI GenInfo Identifier:	30912745
NCBI Gene ID:	29078
NCBI Accession:	Q9P032.1
UniProt Secondary Accession:	Q9P032,B2R4J5,
UniProt Related Accession:	Q9P032
Molecular Weight:	20,266 Da
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase complex assembly factor 4
NCBI Official Symbol:	NDUFAF4
NCBI Official Synonym Symbols:	My013; C6orf66; HRPAP20; HSPC125; bA22L21.1
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
UniProt Synonym Protein Names:	Hormone-regulated proliferation-associated protein of 20 kDa
Protein Family:	NADH dehydrogenase
UniProt Gene Name:	NDUFAF4
UniProt Entry Name:	NDUF4_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFAF4 antibody (CAB14345) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Mouse heart using NDUFAF4 Rabbit pAb (CAB14345) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat heart using NDUFAF4 Rabbit pAb (CAB14345) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human liver cancer using NDUFAF4 Rabbit pAb (CAB14345) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using NDUFAF4 antibody (CAB14345) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)