Anti-NDUFAF2 Antibody (CAB14296)
- SKU:
- CAB14296
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NDUFAF2 Antibody |
| 抗体コード: | CAB14296 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human NDUFAF2 (NP_777549.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HepG2, HeLa, SH-SY5Y, Rat heart |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-169 of human NDUFAF2 (NP_777549.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGWS QDLF RALW RSLS REVK EHVG TDQF GNKY YYIP QYKN WRGQ TIRE KRIV EAAN KKEV DYEA GDIP TEWE AWIR RTRK TPPT MEEI LKNE KHRE EIKI KSQD FYEK EKLL SKET SEEL LPPP VQTQ IKGH ASAP YFGK EEPS VAPS STGK TFQP GSWM PRDG KSHN Q |
| 遺伝子ID: | 91942 |
| Uniprot: | Q8N183 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 19kDa |
| 観察された分子量: | 20kDa |
| 同義語: | NDUFAF2, B17.2L, MMTN, NDUFA12L, mimitin |
| バックグラウンド: | NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency. |
| UniProt Protein Function: | NDUFAF2: Acts as a molecular chaperone for mitochondrial complex I assembly. Defects in NDUFAF2 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFA12 subunit family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 5q12.1 Cellular Component: mitochondrion Molecular Function:NADH dehydrogenase (ubiquinone) activity Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency |
| NCBI Summary: | NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8N183 |
| NCBI GenInfo Identifier: | 67461055 |
| NCBI Gene ID: | 91942 |
| NCBI Accession: | Q8N183.1 |
| UniProt Secondary Accession: | Q8N183,A8K5I1, |
| UniProt Related Accession: | Q8N183 |
| Molecular Weight: | 169 |
| NCBI Full Name: | Mimitin, mitochondrial |
| NCBI Synonym Full Names: | NADH dehydrogenase (ubiquinone) complex I, assembly factor 2 |
| NCBI Official Symbol: | NDUFAF2 |
| NCBI Official Synonym Symbols: | MMTN; B17.2L; mimitin; NDUFA12L |
| NCBI Protein Information: | mimitin, mitochondrial; NDUFA12-like protein; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2 |
| UniProt Protein Name: | Mimitin, mitochondrial |
| UniProt Synonym Protein Names: | B17.2-like; B17.2L; Myc-induced mitochondrial protein; MMTN; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2; NDUFA12-like protein |
| Protein Family: | Mimitin |
| UniProt Gene Name: | NDUFAF2 |
| UniProt Entry Name: | MIMIT_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFAF2 antibody (CAB14296) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using NDUFAF2 antibody (CAB14296) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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