Anti-NDUFAF1 Antibody (CAB15835)
- SKU:
- CAB15835
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-NDUFAF1 Antibody |
| 抗体コード: | CAB15835 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_057097.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa, 293T, A-549, Mouse heart, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human NDUFAF1 (NP_057097.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MALV HKLL RGTY FLRK FSKP TSAL YPFL GIRF AEYS SSLQ KPVA SPGK ASSQ RKTE GDLQ GDHQ KEVA LDIT SSEE KPDV SFDK AIRD EAIY HFRL LKDE IVDH WRGP EGHP LHEV LLEQ AKVV WQFR GKED LDKW TVTS DKTI GGRS EVFL KMGK NNQS ALLY GTLS SEAP QDGE STRS GYCA MISR IPRG AFER KMSY DWSQ FNTL YLRV RGDG RPWM VNIK EDTD FFQR TNQM YSYF MFTR GGPY WQEV KIPF SKFF FSNR GRIR DVQH ELPL DKIS SIGF TLAD KVDG PFFL EIDF IGVF TDPA HTEE FAYE NSPE LNPR LFK |
| 遺伝子ID: | 51103 |
| Uniprot: | Q9Y375 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 37kDa |
| 観察された分子量: | 38kDa |
| 同義語: | NDUFAF1, CGI-65, CGI65, CIA30 |
| バックグラウンド: | This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. |
| UniProt Protein Function: | NDUFAF1: Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Belongs to the CIA30 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 15q11.2-q21.3 Cellular Component: cytoplasm; mitochondrial inner membrane Molecular Function:protein binding Biological Process: mitochondrial respiratory chain complex I assembly Disease: Mitochondrial Complex I Deficiency |
| NCBI Summary: | This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q9Y375 |
| NCBI GenInfo Identifier: | 21542405 |
| NCBI Gene ID: | 51103 |
| NCBI Accession: | Q9Y375.2 |
| UniProt Secondary Accession: | Q9Y375,Q9BVZ5, |
| UniProt Related Accession: | Q9Y375 |
| Molecular Weight: | 37,764 Da |
| NCBI Full Name: | Complex I intermediate-associated protein 30, mitochondrial |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase complex assembly factor 1 |
| NCBI Official Symbol: | NDUFAF1 |
| NCBI Official Synonym Symbols: | CGI65; CIA30; CGI-65 |
| NCBI Protein Information: | complex I intermediate-associated protein 30, mitochondrial |
| UniProt Protein Name: | Complex I intermediate-associated protein 30, mitochondrial |
| UniProt Synonym Protein Names: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 |
| UniProt Gene Name: | NDUFAF1 |
| UniProt Entry Name: | CIA30_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFAF1 antibody (CAB15835) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 35s. |
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