Anti-NDC80 Antibody (CAB14102)
- SKU:
- CAB14102
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-NDC80 Antibody |
| 抗体コード: | CAB14102 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human NDC80 (NP_006092.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | K-562, Lovo, MCF7, 22Rv1, THP-1, 293T, Mouse testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 343-642 of human NDC80 (NP_006092.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | ETIK QENT RLQN IIDN QKYS VADI ERIN HERN ELQQ TINK LTKD LEAE QQKL WNEE LKYA RGKE AIET QLAE YHKL ARKL KLIP KGAE NSKG YDFE IKFN PEAG ANCL VKYR AQVY VPLK ELLN ETEE EINK ALNK KMGL EDTL EQLN AMIT ESKR SVRT LKEE VQKL DDLY QQKI KEAE EEDE KCAS ELES LEKH KHLL ESTV NQGL SEAM NELD AVQR EYQL VVQT TTEE RRKV GNNL QRLL EMVA THVG SVEK HLEE QIAK VDRE YEEC MSED LSEN IKEI RDKY EKKA TLIK SSEE |
| 遺伝子ID: | 10403 |
| Uniprot: | O14777 |
| セルラーロケーション: | Chromosome, Nucleus, centromere, kinetochore |
| 計算された分子量: | 73kDa |
| 観察された分子量: | 74kDa |
| 同義語: | NDC80, HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80 |
| バックグラウンド: | This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. |
| UniProt Protein Function: | HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 18p11.32 Cellular Component: chromosome, pericentric region; cytosol; kinetochore; membrane; nucleus Molecular Function:identical protein binding; protein binding; structural constituent of cytoskeleton Biological Process: attachment of spindle microtubules to kinetochore; attachment of spindle microtubules to kinetochore during mitosis; chromosome segregation; establishment of mitotic spindle orientation; mitosis; mitotic sister chromatid segregation; mitotic spindle organization and biogenesis; sister chromatid cohesion |
| NCBI Summary: | This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011] |
| UniProt Code: | O14777 |
| NCBI GenInfo Identifier: | 74735330 |
| NCBI Gene ID: | 10403 |
| NCBI Accession: | O14777.1 |
| UniProt Secondary Accession: | O14777,Q6PJX2, |
| UniProt Related Accession: | O14777 |
| Molecular Weight: | 73,913 Da |
| NCBI Full Name: | Kinetochore protein NDC80 homolog |
| NCBI Synonym Full Names: | NDC80, kinetochore complex component |
| NCBI Official Symbol: | NDC80 |
| NCBI Official Synonym Symbols: | HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80 |
| NCBI Protein Information: | kinetochore protein NDC80 homolog |
| UniProt Protein Name: | Kinetochore protein NDC80 homolog |
| UniProt Synonym Protein Names: | Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC |
| Protein Family: | Kinetochore protein |
| UniProt Gene Name: | NDC80 |
| UniProt Entry Name: | NDC80_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDC80 antibody (CAB14102) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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