Anti-NCL Antibody (CAB5904)
- SKU:
- CAB5904
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-NCL Antibody |
| 抗体コード: | CAB5904 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 461-710 of human NCL (NP_005372.2). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, A-549, THP-1 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 461-710 of human NCL (NP_005372.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LYYT GEKG QNQD YRGG KNST WSGE SKTL VLSN LSYS ATEE TLQE VFEK ATFI KVPQ NQNG KSKG YAFI EFAS FEDA KEAL NSCN KREI EGRA IRLE LQGP RGSP NARS QPSK TLFV KGLS EDTT EETL KESF DGSV RARI VTDR ETGS SKGF GFVD FNSE EDAK AAKE AMED GEID GNKV TLDW AKPK GEGG FGGR GGGR GGFG GRGG GRGG RGGF GGRG RGGF GGRG GFRG GRGG GGDH KPQG KKTK FE |
| 遺伝子ID: | 4691 |
| Uniprot: | P19338 |
| セルラーロケーション: | Cytoplasm, Nucleus, nucleolus |
| 計算された分子量: | 76kDa |
| 観察された分子量: | 110kDa |
| 同義語: | NCL, C23, Nsr1, nucleolin |
| バックグラウンド: | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. |
| UniProt Protein Function: | NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly. |
| UniProt Protein Details: | Protein type:Helicase; RNA-binding; Nucleolus; Nuclear receptor co-regulator; DNA-binding Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: cell cortex; membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex Molecular Function:identical protein binding; protein binding; protein C-terminus binding; RNA binding; telomeric DNA binding Biological Process: angiogenesis |
| NCBI Summary: | Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P19338 |
| NCBI GenInfo Identifier: | 90110781 |
| NCBI Gene ID: | 4691 |
| NCBI Accession: | P19338.3 |
| UniProt Secondary Accession: | P19338,Q53SK1, Q8NB06, Q9UCF0, Q9UDG1, |
| UniProt Related Accession: | P19338 |
| Molecular Weight: | 76,614 Da |
| NCBI Full Name: | Nucleolin |
| NCBI Synonym Full Names: | nucleolin |
| NCBI Official Symbol: | NCL |
| NCBI Official Synonym Symbols: | C23 |
| NCBI Protein Information: | nucleolin |
| UniProt Protein Name: | Nucleolin |
| UniProt Synonym Protein Names: | Protein C23 |
| Protein Family: | Nucleolin |
| UniProt Gene Name: | NCL |
| UniProt Entry Name: | NUCL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NCL antibody (CAB5904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using NCL Rabbit pAb (CAB5904) at dilution of 1:100 (40x lens). |
 | Confocal immunofluorescence analysis of U2OS cells using NCL Polyclonal Antibody (CAB5904) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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