Anti-NCAPG Antibody (CAB13123)
- SKU:
- CAB13123
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-NCAPG Antibody |
| 抗体コード: | CAB13123 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 610-700 of human NCAPG (NP_071741.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | HeLa, 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 610-700 of human NCAPG (NP_071741.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | CCGL QNQD FARK HFVL LLQV LQID DVTI KISA LKAI FDQL MTFG IEPF KTKK IKTL HCEG TEIN SDDE QESK EVEE TATA KNVL KLLS DFL |
| 遺伝子ID: | 64151 |
| Uniprot: | Q9BPX3 |
| セルラーロケーション: | Chromosome, Cytoplasm, Nucleus |
| 計算された分子量: | 114kDa |
| 観察された分子量: | 114kDa |
| 同義語: | NCAPG, CAPG, CHCG, NY-MEL-3, YCG1 |
| バックグラウンド: | This gene encodes a subunit of the condensin complex, which is responsible for the condensation and stabilization of chromosomes during mitosis and meiosis. Phosphorylation of the encoded protein activates the condensin complex. There are pseudogenes for this gene on chromosomes 8 and 15. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | NCAPG: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Belongs to the CND3 (condensin subunit 3) family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 4p15.33 Cellular Component: centrosome; membrane; cytoplasm; condensin complex; nucleus; cytosol; actin cytoskeleton Molecular Function:protein binding Biological Process: cell division; mitotic chromosome condensation; mitotic cell cycle |
| NCBI Summary: | This gene encodes a subunit of the condensin complex, which is responsible for the condensation and stabilization of chromosomes during mitosis and meiosis. Phosphorylation of the encoded protein activates the condensin complex. There are pseudogenes for this gene on chromosomes 8 and 15. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| UniProt Code: | Q9BPX3 |
| NCBI GenInfo Identifier: | 30172941 |
| NCBI Gene ID: | 64151 |
| NCBI Accession: | Q9BPX3.1 |
| UniProt Secondary Accession: | Q9BPX3,Q3MJE0, Q96SV9, Q9BUR3, Q9BVY1, Q9H914, Q9H9Z6 Q9HBI9, |
| UniProt Related Accession: | Q9BPX3 |
| Molecular Weight: | 114,334 Da |
| NCBI Full Name: | Condensin complex subunit 3 |
| NCBI Synonym Full Names: | non-SMC condensin I complex, subunit G |
| NCBI Official Symbol: | NCAPG |
| NCBI Official Synonym Symbols: | CAPG; CHCG; YCG1; NY-MEL-3 |
| NCBI Protein Information: | condensin complex subunit 3; XCAP-G homolog; condensin subunit CAP-G; melanoma antigen NY-MEL-3; chromosome-associated protein G; chromosome condensation protein G |
| UniProt Protein Name: | Condensin complex subunit 3 |
| UniProt Synonym Protein Names: | Chromosome-associated protein G; Condensin subunit CAP-G; hCAP-G; Melanoma antigen NY-MEL-3; Non-SMC condensin I complex subunit G; XCAP-G homolog |
| UniProt Gene Name: | NCAPG |
| UniProt Entry Name: | CND3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NCAPG antibody (CAB13123) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |