Anti-Myosin-6 抗体 (CAB9516)

抗体名:	Anti-Myosin-6 Antibody
抗体コード:	CAB9516
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IF
反応性:	Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MYH6 (NP_002462.2).

申し込み:	WB IF
推奨希釈:	WB 1:500 - 1:2000 IF 1:50 - 1:200
反応性:	Mouse, Rat
ポジティブサンプル:	Mouse skeletal muscle

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human MYH6 (NP_002462.2).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MTDA QMAD FGAA AQYL RKSE KERL EAQT RPFD IRTE CFVP DDKE EFVK AKIL SREG GKVI AETE NGKT VTVK EDQV LQQN PPKF DKIE DMAM LTFL HEPA VLFN LKER YAAW MIYT YSGL FCVT VNPY KWLP VYNA EVVA AYRG KKRS EAPP HIFS ISDN AYQY MLTD RENQ SILI TGES GAGK TVNT KRVI QYFA SIAA IGDR GKKD NANA NKGT LEDQ IIQA NPAL EAFG NAKT VRND NSSR FGKF IR
遺伝子ID:	4624
Uniprot:	P13533
セルラーロケーション:	Cytoplasm, myofibril
計算された分子量:	223kDa
観察された分子量:	255kDa

同義語:

MYH6, ASD3, CMD1EE, CMH14, MYHC, MYHCA, SSS3, alpha-MHC, myosin-6

バックグラウンド:

Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located approximately 4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3.

UniProt Protein Function:	MYH6: Muscle contraction. Defects in MYH6 are the cause of atrial septal defect type 3 (ASD3). ASD3 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. Defects in MYH6 are the cause of familial hypertrophic cardiomyopathy type 14 (CMH14). It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations,and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Defects in MYH6 are the cause of cardiomyopathy dilated type 1EE (CMD1EE). It is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in MYH6 are the cause of susceptibility to sick sinus syndrome type 3 (SSS3). The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors. MYH6 variations are associated with susceptibility to sick sinus syndrome (PubMed:21378987). The lifetime risk of being diagnosed with sick sinus syndrome is higher for carriers of variant p.Arg721Trp than for non-carriers (PubMed:21378987).
UniProt Protein Details:	Protein type:Motor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 14q12 Cellular Component: cytosol; muscle myosin complex; myofibril; myosin complex; sarcomere Molecular Function:actin-dependent ATPase activity; ATPase activity; microfilament motor activity; myosin phosphatase activity; protein kinase binding Biological Process: adult heart development; ATP metabolic process; atrial cardiac muscle morphogenesis; cardiac muscle contraction; cardiac muscle fiber development; in utero embryonic development; muscle contraction; muscle filament sliding; myofibril assembly; regulation of ATPase activity; regulation of blood pressure; regulation of heart contraction; regulation of heart rate; regulation of the force of heart contraction; sarcomere organization; striated muscle contraction; ventricular cardiac muscle morphogenesis; visceral muscle development Disease: Atrial Septal Defect 3; Cardiomyopathy, Dilated, 1ee; Cardiomyopathy, Familial Hypertrophic, 1; Cardiomyopathy, Familial Hypertrophic, 14; Sick Sinus Syndrome 3, Susceptibility To
NCBI Summary:	Cardiac muscle myosin is a hexamer consisting of two heavy chain subunits, two light chain subunits, and two regulatory subunits. This gene encodes the alpha heavy chain subunit of cardiac myosin. The gene is located ~4kb downstream of the gene encoding the beta heavy chain subunit of cardiac myosin. Mutations in this gene cause familial hypertrophic cardiomyopathy and atrial septal defect 3. [provided by RefSeq, Mar 2010]
UniProt Code:	P13533
NCBI GenInfo Identifier:	317373582
NCBI Gene ID:	4624
NCBI Accession:	P13533.5
UniProt Secondary Accession:	P13533,Q13943, Q14906, Q14907, A2RTX1, D9YZU2,
UniProt Related Accession:	P13533
Molecular Weight:	223,735 Da
NCBI Full Name:	Myosin-6
NCBI Synonym Full Names:	myosin heavy chain 6
NCBI Official Symbol:	MYH6
NCBI Official Synonym Symbols:	ASD3; MYHC; SSS3; CMH14; MYHCA; CMD1EE; alpha-MHC
NCBI Protein Information:	myosin-6
UniProt Protein Name:	Myosin-6
UniProt Synonym Protein Names:	Myosin heavy chain 6; Myosin heavy chain, cardiac muscle alpha isoform; MyHC-alpha
Protein Family:	Myosin
UniProt Gene Name:	MYH6
UniProt Entry Name:	MYH6_HUMAN

	Western blot analysis of extracts of mouse skeletal muscle, using MYH6 antibody (CAB9516). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of mouse heart using MYH6 antibody (CAB9516) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat heart using MYH6 antibody (CAB9516) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-MYH6 Antibody (CAB12964)

MYH6 Antibody (PACO07449)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)