Anti-MYO6 Antibody (CAB13033)
- SKU:
- CAB13033
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MYO6 Antibody |
| 抗体コード: | CAB13033 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1016-1285 of human MYO6 (NP_004990.3). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HT-29, Mouse brain, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1016-1285 of human MYO6 (NP_004990.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | IAQS EAEL ISDE AQAD LALR RNDG TRPK MTPE QMAK EMSE FLSR GPAV LATK AAAG TKKY DLSK WKYA ELRD TINT SCDI ELLA ACRE EFHR RLKV YHAW KSKN KKRN TETE QRAP KSVT DYDF APFL NNSP QQNP AAQI PARQ REIE MNRQ QRFF RIPF IRPA DQYK DPQS KKKG WWYA HFDG PWIA RQME LHPD KPPI LLVA GKDD MEMC ELNL EETG LTRK RGAE ILPR QFEE IWER CGGI QYLQ NAIE SRQA RPTY ATAM LQSL LK |
| 遺伝子ID: | 4646 |
| Uniprot: | Q9UM54 |
| セルラーロケーション: | Cell projection, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus, Peripheral membrane protein, Peripheral membrane protein, clathrin-coated pit, clathrin-coated vesicle membrane, perinuclear region, ruffle membrane, trans-Golgi network membrane |
| 計算された分子量: | 145kDa/146kDa/148kDa/149kDa |
| 観察された分子量: | 150kDa |
| 同義語: | MYO6, DFNA22, DFNB37, Myo6-007, Myo6-008, myosin VI |
| バックグラウンド: | This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | MYO6: a molecular motor involved in intracellular vesicle and organelle transport. It is one of the so-called unconventional myosins. |
| UniProt Protein Details: | Protein type:Actin-binding; Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 6q13 Cellular Component: cell cortex; clathrin-coated endocytic vesicle; cytoplasm; cytoplasmic membrane-bound vesicle; cytosol; DNA-directed RNA polymerase II, holoenzyme; endocytic vesicle; filamentous actin; Golgi apparatus; lysosomal membrane; membrane; nuclear membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; ruffle; unconventional myosin complex Molecular Function:actin binding; actin filament binding; ADP binding; calmodulin binding; motor activity; protein binding Biological Process: actin filament-based movement; DNA damage response, signal transduction by p53 class mediator; endocytosis; intracellular protein transport; positive regulation of transcription from RNA polymerase II promoter; regulation of secretion Disease: Deafness, Autosomal Dominant 22; Deafness, Autosomal Recessive 37 |
| NCBI Summary: | This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014] |
| UniProt Code: | Q9UM54 |
| NCBI GenInfo Identifier: | 122065628 |
| NCBI Gene ID: | 4646 |
| NCBI Accession: | Q9UM54.4 |
| UniProt Secondary Accession: | Q9UM54,Q5TEM5, Q5TEM6, Q5TEM7, Q9BZZ7, Q9UEG2, A6H8V4 E1P540, |
| UniProt Related Accession: | Q9UM54 |
| Molecular Weight: | 148,659 Da |
| NCBI Full Name: | Unconventional myosin-VI |
| NCBI Synonym Full Names: | myosin VI |
| NCBI Official Symbol: | MYO6 |
| NCBI Official Synonym Symbols: | DFNA22; DFNB37 |
| NCBI Protein Information: | unconventional myosin-VI |
| UniProt Protein Name: | Unconventional myosin-VI |
| UniProt Synonym Protein Names: | Unconventional myosin-6 |
| Protein Family: | Unconventional myosin |
| UniProt Gene Name: | MYO6 |
| UniProt Entry Name: | MYO6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYO6 antibody (CAB13033) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using MYO6 antibody (CAB13033) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using MYO6 antibody (CAB13033) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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