Anti-MYLIP Antibody (CAB6166)
- SKU:
- CAB6166
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Frequently bought together:
Description
| 抗体名: | Anti-MYLIP Antibody |
| 抗体コード: | CAB6166 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 266-445 of human MYLIP (NP_037394.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat, Mouse kidney, Mouse liver, Mouse spleen, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 266-445 of human MYLIP (NP_037394.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RAIT ETHA FYRC DTVT SAVM MQYS RDLK GHLA SLFL NENI NLGK KYVF DIKR TSKE VYDH ARRA LYNA GVVD LVSR NNQS PSHS PLKS SESS MNCS SCEG LSCQ QTRV LQEK LRKL KEAM LCMV CCEE EINS TFCP CGHT VCCE SCAA QLQS CPVC RSRV EHVQ HVYL PTHT SLLN LTVI |
| 遺伝子ID: | 29116 |
| Uniprot: | Q8WY64 |
| セルラーロケーション: | Cell membrane, Cytoplasm, Peripheral membrane protein |
| 計算された分子量: | 14kDa/49kDa |
| 観察された分子量: | 50kDa |
| 同義語: | MYLIP, IDOL, MIR |
| バックグラウンド: | The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. |
| UniProt Protein Function: | MYLIP: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Motility/polarity/chemotaxis; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: extrinsic to membrane; cytoskeleton; cytoplasm; plasma membrane; intracellular Molecular Function:protein binding; zinc ion binding; cytoskeletal protein binding; ubiquitin-protein ligase activity; ligase activity Biological Process: nervous system development; cholesterol homeostasis; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein ubiquitination; regulation of low-density lipoprotein receptor catabolic process; cell motility |
| NCBI Summary: | The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8WY64 |
| NCBI GenInfo Identifier: | 38788243 |
| NCBI Gene ID: | 29116 |
| NCBI Accession: | NP_037394.2 |
| UniProt Secondary Accession: | Q8WY64,Q5TIA4, Q9BU73, Q9NRL9, Q9UHE7, |
| UniProt Related Accession: | Q8WY64 |
| Molecular Weight: | 49,910 Da |
| NCBI Full Name: | E3 ubiquitin-protein ligase MYLIP |
| NCBI Synonym Full Names: | myosin regulatory light chain interacting protein |
| NCBI Official Symbol: | MYLIP |
| NCBI Official Synonym Symbols: | MIR; IDOL |
| NCBI Protein Information: | E3 ubiquitin-protein ligase MYLIP; band 4.1 superfamily member BZF1; inducible degrader of the LDL-receptor; cellular modulator of immune recognition (c-MIR); myosin regulatory light chain-interacting protein; E3 ubiquitin ligase-inducible degrader of the |
| UniProt Protein Name: | E3 ubiquitin-protein ligase MYLIP |
| UniProt Synonym Protein Names: | Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein |
| UniProt Gene Name: | MYLIP |
| UniProt Entry Name: | MYLIP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYLIP antibody (CAB6166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human prostate using MYLIP antibody (CAB6166) at dilution of 1:100 (40x lens). |
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