Anti-MYL2 Antibody (CAB5473)
- SKU:
- CAB5473
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MYL2 Antibody |
| 抗体コード: | CAB5473 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | MCF7, Mouse skeletal muscle |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MYL2 (NP_000423.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAPK KAKK RAGG ANSN VFSM FEQT QIQE FKEA FTIM DQNR DGFI DKND LRDT FAAL GRVN VKNE EIDE MIKE APGP INFT VFLT MFGE KLKG ADPE ETIL NAFK VFDP EGKG VLKA DYVR EMLT TQAE RFSK EEVD QMFA AFPP DVTG NLDY KNLV HIIT HGEE KD |
| 遺伝子ID: | 4633 |
| Uniprot: | P10916 |
| セルラーロケーション: | A band, Cytoplasm, myofibril, sarcomere |
| 計算された分子量: | 18kDa |
| 観察された分子量: | 16kDa |
| 同義語: | MYL2, CMH10, MLC-2s/v, MLC2 |
| バックグラウンド: | Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. |
| UniProt Protein Function: | MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. |
| UniProt Protein Details: | Protein type:Contractile Chromosomal Location of Human Ortholog: 12q24.11 Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton Molecular Function:actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; cardiac muscle contraction; post-embryonic development Disease: Cardiomyopathy, Familial Hypertrophic, 10 |
| NCBI Summary: | Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P10916 |
| NCBI GenInfo Identifier: | 6166556 |
| NCBI Gene ID: | 4633 |
| NCBI Accession: | P10916.3 |
| UniProt Secondary Accession: | P10916,Q16123, |
| UniProt Related Accession: | P10916 |
| Molecular Weight: | 166 |
| NCBI Full Name: | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform |
| NCBI Synonym Full Names: | myosin, light chain 2, regulatory, cardiac, slow |
| NCBI Official Symbol: | MYL2 |
| NCBI Official Synonym Symbols: | MLC2; CMH10 |
| NCBI Protein Information: | myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow |
| UniProt Protein Name: | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform |
| Protein Family: | Myosin regulatory |
| UniProt Gene Name: | MYL2 |
| UniProt Entry Name: | MLRV_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYL2 antibody (CAB5473) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of rat heart using MYL2 Rabbit pAb (CAB5473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse heart using MYL2 Rabbit pAb (CAB5473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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