Anti-MYH2 Antibody (CAB15292)
- SKU:
- CAB15292
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MYH2 Antibody |
| 抗体コード: | CAB15292 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MYH2 (NP_001093582.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse skeletal muscle, Rat skeletal muscle |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human MYH2 (NP_001093582.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | SFKN KLYD QHLG KSAN FQKP KVVK GKAE AHFA LIHY AGVV DYNI TGWL EKNK DPLN ETVV GLYQ KSAM KTLA QLFS GAQT AEGE GAGG GAKK GGKK KGSS F |
| 遺伝子ID: | 4620 |
| Uniprot: | Q9UKX2 |
| セルラーロケーション: | Cytoplasm, myofibril |
| 計算された分子量: | 79kDa/223kDa |
| 観察された分子量: | 223kDa |
| 同義語: | MYH2, IBM3, MYH2A, MYHSA2, MYHas8, MYPOP, MyHC-2A, MyHC-IIa, myosin-2 |
| バックグラウンド: | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| UniProt Protein Function: | MYH2: Muscle contraction. Required for cytoskeleton organization. Defects in MYH2 are the cause of inclusion body myopathy type 3 (IBM3). Hereditary inclusion body myopathies constitute a group of neuromuscular disorders characterized by slowly progressive distal and proximal weakness and a typical muscle pathology including rimmed vacuoles and filamentous inclusions. IBM3 is a variant of hereditary inclusion body myopathies and is characterized by autosomal dominant myopathy with joint contracture, ophthalmoplegia and rimmed vacuoles. Morphological analysis of muscle biopsies from patients indicate that the type 2A fibers frequently were abnormal, whereas other fiber types appeared normal. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: Golgi apparatus; sarcomere; protein complex; myofibril; contractile ring; muscle myosin complex; intercellular junction; cytosol; A band Molecular Function:microfilament motor activity; calmodulin binding; protein binding; actin binding; ATP binding Biological Process: muscle contraction; plasma membrane repair; metabolic process; innate immune response; response to activity; muscle filament sliding Disease: Inclusion Body Myopathy 3, Autosomal Dominant |
| NCBI Summary: | Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009] |
| UniProt Code: | Q9UKX2 |
| NCBI GenInfo Identifier: | 13431716 |
| NCBI Gene ID: | 4620 |
| NCBI Accession: | Q9UKX2.1 |
| UniProt Secondary Accession: | Q9UKX2,Q14322, Q16229, Q567P6, Q86T56, A0AVL4, |
| UniProt Related Accession: | Q9UKX2 |
| Molecular Weight: | 1941 |
| NCBI Full Name: | Myosin-2 |
| NCBI Synonym Full Names: | myosin, heavy chain 2, skeletal muscle, adult |
| NCBI Official Symbol: | MYH2 |
| NCBI Official Synonym Symbols: | IBM3; MYH2A; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa |
| NCBI Protein Information: | myosin-2; myosin heavy chain 2; myosin heavy chain 2a; myosin heavy chain IIa; fast 2a myosin heavy chain; type IIA myosin heavy chain; myosin heavy chain, skeletal muscle, adult 2; inclusion body myopathy 3, autosomal dominant; myosin, heavy polypeptide 2, skeletal muscle, adult |
| UniProt Protein Name: | Myosin-2 |
| UniProt Synonym Protein Names: | Myosin heavy chain 2; Myosin heavy chain 2a; MyHC-2a; Myosin heavy chain IIa; MyHC-IIa; Myosin heavy chain, skeletal muscle, adult 2 |
| UniProt Gene Name: | MYH2 |
| UniProt Entry Name: | MYH2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MYH2 antibody (CAB15292) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s. |
 | Immunofluorescence analysis of mouse skeletal muscle using MYH2 Polyclonal Antibody (CAB15292) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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