Anti-MRPL30 Antibody (CAB16529)
- SKU:
- CAB16529
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MRPL30 Antibody |
| 抗体コード: | CAB16529 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human MRPL30 (NP_660213.1). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | HeLa, U-251MG, 293T |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human MRPL30 (NP_660213.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAGI LRLV VQWP PGRL QTVT KGVE SLIC TDWI RHKF TRSR IPEK VFQA SPED HEKY GGDP QNPH KLHI VTRI KSTR RRPY WEKD IIKM LGLE KAHT PQVH KNIP SVNA KLKV VKHL IRIK PLKL PQGL PAEE NMSN TCLK STGE LVVQ WHLK PVEQ KAHE S |
| 遺伝子ID: | 51263 |
| Uniprot: | Q8TCC3 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 15kDa/18kDa/21kDa |
| 観察された分子量: | 18kDa |
| 同義語: | MRPL30, L28MT, L30MT, MRP-L28, MRP-L30, MRPL28, MRPL28M, RPML28 |
| バックグラウンド: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene. |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene. [provided by RefSeq, Mar 2011] |
| UniProt Code: | Q8TCC3 |
| NCBI GenInfo Identifier: | 21735604 |
| NCBI Gene ID: | 51263 |
| NCBI Accession: | NP_660213.1 |
| UniProt Secondary Accession: | Q8TCC3,Q0D2Q7, Q6ZTP4, Q96Q69, Q9P0N0, A6NIC6, D3DVI0 D3DVI3, |
| UniProt Related Accession: | Q8TCC3 |
| Molecular Weight: | 17 |
| NCBI Full Name: | 39S ribosomal protein L30, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L30 |
| NCBI Official Symbol: | MRPL30 |
| NCBI Official Synonym Symbols: | L28MT; L30MT; MRPL28; RPML28; MRP-L28; MRP-L30; MRPL28M |
| NCBI Protein Information: | 39S ribosomal protein L30, mitochondrial |
| UniProt Protein Name: | 39S ribosomal protein L30, mitochondrial |
| UniProt Synonym Protein Names: | 39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; Mitochondrial large ribosomal subunit protein uL30m |
| UniProt Gene Name: | MRPL30 |
 | Western blot analysis of extracts of various cell lines, using MRPL30 Rabbit pAb (CAB16529) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded Human breast cancer using MRPL30 Rabbit pAb (CAB16529) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat ovary using MRPL30 Rabbit pAb (CAB16529) at dilution of 1:100 (40x lens). |
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