Anti-MRPL23 抗体 (CAB16424)

抗体名:	Anti-MRPL23 Antibody
抗体コード:	CAB16424
抗体サイズ:	20uL, 50uL, 100uL
申し込み:	WB IHC IF
反応性:	Human, Mouse, Rat
宿主種:	Rabbit
免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human MRPL23 (NP_066957.3).

申し込み:	WB IHC IF
推奨希釈:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
反応性:	Human, Mouse, Rat
ポジティブサンプル:	HeLa, U-251MG, A-549, Mouse liver, Mouse lung, Mouse brain, Rat heart, Rat liver, Rat kidney

免疫原:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-153 of human MRPL23 (NP_066957.3).
精製方法:	Affinity purification
ストレージバッファ:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:	IgG
順序:	MARN VVYP LYRL GGPQ LRVF RTNF FIQL VRPG VAQP EDTV QFRI PMEM TRVD LRNY LEGI YNVP VAAV RTRV QHGS NKRR DHRN VRIK KPDY KVAY VQLA HGQT FTFP DLFP EKDE SPEG SAAD DLYS MLEE ERQQ RQSS DPRR GGVP SWFG L
遺伝子ID:	6150
Uniprot:	Q16540
セルラーロケーション:	Mitochondrion
計算された分子量:	17kDa
観察された分子量:	15kDa

同義語:

MRPL23, L23MRP, RPL23, RPL23L

バックグラウンド:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11.

UniProt Protein Function:	MRPL23: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Mitochondrial; Ribosomal; RNA-binding Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: mitochondrion; mitochondrial inner membrane; nucleolus; mitochondrial large ribosomal subunit Molecular Function:structural constituent of ribosome; RNA binding; nucleotide binding Biological Process: mitochondrial translation; translation; organelle organization and biogenesis
UniProt Code:	Q16540
NCBI GenInfo Identifier:
NCBI Gene ID:	1433
NCBI Accession:	NP_001306.2
UniProt Secondary Accession:	Q16540,Q96Q71, A8MT29,
Molecular Weight:	17,781 Da
NCBI Full Name:
NCBI Synonym Full Names:	choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity)
NCBI Official Symbol:	CSE
NCBI Official Synonym Symbols:	DYT9
UniProt Protein Name:	39S ribosomal protein L23, mitochondrial
UniProt Synonym Protein Names:	L23 mitochondrial-related protein; Ribosomal protein L23-like
Protein Family:	Caffeoylshikimate esterase
UniProt Gene Name:	MRPL23
UniProt Entry Name:	RM23_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPL23 antibody (CAB16424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human thyroid cancer using MRPL23 antibody (CAB16424) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MRPL23 antibody (CAB16424) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using MRPL23 antibody (CAB16424) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

MRPL23 Antibody (PACO10580)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)