Anti-MRPL22 Antibody (CAB14586)
- SKU:
- CAB14586
- Product type:
- Antibody
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MRPL22 Antibody |
| 抗体コード: | CAB14586 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 97-206 of human MRPL22 (NP_054899.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Mouse, Rat |
| ポジティブサンプル: | Mouse brain, Mouse heart, Mouse kidney, Rat lung |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 97-206 of human MRPL22 (NP_054899.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | LAQL EFND KKGA KIIK EVLL EAQD MAVR DHNV EFRS NLYI AEST SGRG QCLK RIRY HGRG RFGI MEKV YCHY FVKL VEGP PPPP EPPK TAVA HAKE YIQQ LRSR TIVH TL |
| 遺伝子ID: | 29093 |
| Uniprot: | Q9NWU5 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 13kDa/14kDa/23kDa |
| 観察された分子量: | 24kDa |
| 同義語: | MRPL22, HSPC158, L22mt, MRP-L22, MRP-L25, RPML25 |
| バックグラウンド: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MRPL22: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Translation Chromosomal Location of Human Ortholog: 5q33.2 Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrion Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; organelle organization and biogenesis |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NWU5 |
| NCBI GenInfo Identifier: | 74753031 |
| NCBI Gene ID: | 29093 |
| NCBI Accession: | Q9NWU5.1 |
| UniProt Secondary Accession: | Q9NWU5,Q5H9Q1, Q96Q51, Q9P006, A6NGJ8, |
| UniProt Related Accession: | Q9NWU5 |
| Molecular Weight: | 14,509 Da |
| NCBI Full Name: | 39S ribosomal protein L22, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L22 |
| NCBI Official Symbol: | MRPL22 |
| NCBI Official Synonym Symbols: | L22mt; RPML25; HSPC158; MRP-L22; MRP-L25 |
| NCBI Protein Information: | 39S ribosomal protein L22, mitochondrial |
| UniProt Protein Name: | 39S ribosomal protein L22, mitochondrial |
| UniProt Synonym Protein Names: | 39S ribosomal protein L25, mitochondrial; L25mt; MRP-L25 |
| Protein Family: | 39S ribosomal protein |
| UniProt Gene Name: | MRPL22 |
| UniProt Entry Name: | RM22_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MRPL22 antibody (CAB14586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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