Anti-MRPL15 Antibody (CAB14400)
- SKU:
- CAB14400
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MRPL15 Antibody |
| 抗体コード: | CAB14400 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human MRPL15 (NP_054894.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | U-87MG, HeLa, MCF7, A-549, Mouse heart, Mouse kidney, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-296 of human MRPL15 (NP_054894.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAGP LQGG GARA LDLL RGLP RVSL ANLK PNPG SKKP ERRP RGRR RGRK CGRG HKGE RQRG TRPR LGFE GGQT PFYI RIPK YGFN EGHS FRRQ YKPL SLNR LQYL IDLG RVDP SQPI DLTQ LVNG RGVT IQPL KRDY GVQL VEEG ADTF TAKV NIEV QLAS ELAI AAIE KNGG VVTT AFYD PRSL DIVC KPVP FFLR GQPI PKRM LPPE ELVP YYTD AKNR GYLA DPAK FPEA RLEL ARKY GYIL PDIT KDEL FKML CTRK DPRQ IFFG LAPG WVVN MADK KILK PTDE NLLK YYTS |
| 遺伝子ID: | 29088 |
| Uniprot: | Q9P015 |
| セルラーロケーション: | Mitochondrion |
| 計算された分子量: | 33kDa |
| 観察された分子量: | 36kDa |
| 同義語: | MRPL15, HSPC145, L15mt, MRP-L15, MRP-L7, RPML7 |
| バックグラウンド: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. |
| UniProt Protein Function: | MRPL15: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Translation; Mitochondrial; Ribosomal; RNA-binding Chromosomal Location of Human Ortholog: 8q11.2-q13 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial large ribosomal subunit Molecular Function:structural constituent of ribosome Biological Process: mitochondrial translation; organelle organization and biogenesis |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9P015 |
| NCBI GenInfo Identifier: | 7661806 |
| NCBI Gene ID: | 29088 |
| NCBI Accession: | NP_054894.1 |
| UniProt Related Accession: | Q9P015 |
| Molecular Weight: | 33kDa |
| NCBI Full Name: | 39S ribosomal protein L15, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L15 |
| NCBI Official Symbol: | MRPL15 |
| NCBI Official Synonym Symbols: | L15mt; RPML7; MRP-L7; HSPC145; MRP-L15 |
| NCBI Protein Information: | 39S ribosomal protein L15, mitochondrial |
| UniProt Protein Name: | 39S ribosomal protein L15, mitochondrial |
| Protein Family: | 39S ribosomal protein |
| UniProt Gene Name: | MRPL15 |
| UniProt Entry Name: | RM15_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MRPL15 antibody (CAB14400) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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