Anti-MPG Antibody (CAB5410)
- SKU:
- CAB5410
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-MPG Antibody |
| 抗体コード: | CAB5410 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | MCF7, K-562, U-937, Mouse liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MPAR SGAQ FCRR MGQK KQRP ARAG QPHS SSDA AQAP AEQP HSSS DAAQ APCP RERC LGPP TTPG PYRS IYFS SPKG HLTR LGLE FFDQ PAVP LARA FLGQ VLVR RLPN GTEL RGRI VETE AYLG PEDE AAHS RGGR QTPR NRGM FMKP GTLY VYII YGMY FCMN ISSQ GDGA CVLL RALE PLEG LETM RQLR STLR KGTA SRVL KDRE LCSG PSKL CQAL AINK SFDQ RDLA QDEA VWLE RGPL EPSE PAVV AAAR VGVG HAGE WARK PLRF YVRG SPWV SVVD RVAE QDTQ A |
| 遺伝子ID: | 4350 |
| Uniprot: | P29372 |
| セルラーロケーション: | Cytoplasm, Mitochondrion matrix, Nucleus, mitochondrion nucleoid |
| 計算された分子量: | 30kDa/32kDa |
| 観察された分子量: | 37kDa |
| 同義語: | MPG, AAG, ADPG, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16, anpg |
| バックグラウンド: |
| UniProt Protein Function: | MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 3.2.2.21; Hydrolase; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm Molecular Function:protein binding; alkylbase DNA N-glycosylase activity; DNA-3-methyladenine glycosylase I activity; damaged DNA binding Biological Process: DNA dealkylation; base-excision repair, AP site formation; depurination; base-excision repair; DNA repair |
| UniProt Code: | P29372 |
| NCBI GenInfo Identifier: | 206729922 |
| NCBI Gene ID: | 4350 |
| NCBI Accession: | P29372.3 |
| UniProt Secondary Accession: | P29372,Q13770, Q15275, Q15961, Q5J9I4, Q96BZ6, Q96S33 Q9NNX5, G5E9E2, |
| UniProt Related Accession: | P29372 |
| Molecular Weight: | 298 |
| NCBI Full Name: | DNA-3-methyladenine glycosylase |
| NCBI Synonym Full Names: | N-methylpurine-DNA glycosylase |
| NCBI Official Symbol: | MPG |
| NCBI Official Synonym Symbols: | AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1 |
| NCBI Protein Information: | DNA-3-methyladenine glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; proliferation-inducing protein 11; proliferation-inducing protein 16; N-methylpurine-DNA glycosylase, MPG; CRA36.1 (3-methyl-adenine DNA glycosylase); 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p |
| UniProt Protein Name: | DNA-3-methyladenine glycosylase |
| UniProt Synonym Protein Names: | 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase |
| Protein Family: | DNA-3-methyladenine glycosylase |
| UniProt Gene Name: | MPG |
| UniProt Entry Name: | 3MG_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MPG antibody (CAB5410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 40s. |
 | Immunofluorescence analysis of U2OS cells using MPG antibody (CAB5410). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using MPG antibody (CAB5410). Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region. |
 | Immunofluorescence analysis of A549 cells using MPG antibody (CAB5410). |
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