Anti-Mouse anti RFP-Tag Monoclonal Antibody (CABE020)
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- SKU:
- CABE020
- Product type:
- Antibody
- Host Species:
- Mouse
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
Frequently bought together:
Description
| 抗体名: | Anti-Mouse anti RFP-Tag Monoclonal Antibody |
| 抗体コード: | CABE020 |
| 抗体サイズ: | 100 uL |
| 申し込み: | WB IHC IP |
| 反応性: | |
| 宿主種: | Mouse |
| 免疫原: | Recombinant protein of RFP-Tag |
| 申し込み: | WB IHC IP |
| 推奨希釈: | WB 1:2000 - 1:5000 IHC 1:50 - 1:200 IP 1:50 - 1:200 |
| 反応性: | |
| ポジティブサンプル: |
| 免疫原: | Recombinant protein of RFP-Tag |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MASS ENVI TEFM RFKV RMEG TVNG HEFE IEGE GEGR PYEG HNTV KLKV TKGG PLPF AWDI LSPQ FQYG SKVY VKHP ADIP DYKK LSFP EGFK WERV MNFE DGGV ATVT QDSS LQDG CFIY KVKF IGVN FPSD GPVM QKKT MGWE ASTE RLYP RDGV LKGE THKA LKLK DGGH YLVE FKSI YMAK KPVQ LPGY YYVD AKLD ITSH NEDY TIVE QYER TEGR HHLF L |
| 遺伝子ID: | |
| Uniprot: | |
| セルラーロケーション: | |
| 計算された分子量: | 27kDa |
| 観察された分子量: | Refer to Figures |
 | Western blot analysis of recombinant RFP protein using RFP-tag antibody (CABE020) at dilution of 1:10000. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
| 同義語: | RFP, RFP tag, RFP-tag |
| バックグラウンド: | Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes. Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. |