Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)

(No reviews yet) Write a Review
SKU:
CABE003
Product type:
Antibody
Host Species:
Mouse
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
€0.00
Frequently bought together:

Description

system_update_altデータシートsystem_update_alt安全性データシート
抗体名:Anti-Mouse anti His-Tag Monoclonal Antibody
抗体コード:CABE003
抗体サイズ:100 uL
申し込み:WB IF IP
反応性:
宿主種:Mouse
免疫原:A synthetic peptide corresponding to His tag.
申し込み:WB IF IP
推奨希釈:WB 1:2000 - 1:5000 IF 1:50 - 1:200 IP 1:50 - 1:100
反応性:
ポジティブサンプル:293T
免疫原:A synthetic peptide corresponding to His tag.
精製方法:Affinity purification
ストレージバッファ:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:HHHH HH
遺伝子ID:
Uniprot:
セルラーロケーション:
計算された分子量:
観察された分子量:Refer to Figures
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein, using His-Tag antibody (CABE003) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)Western blot analysis of extracts of normal 293T cells and 293T transfected with EPCAM protein, using His-Tag antibody (CABE003) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb (CABE003) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-Mouse anti His-Tag Monoclonal Antibody (CABE003)Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using His-tag antibody (CABE003). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
同義語:His, His tag, His-tag
バックグラウンド:Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
View AllClose

View AllClose

Related Products