Anti-Mouse anti HA-Tag Monoclonal Antibody (CABE008)
- SKU:
- CABE008
- Product type:
- Antibody
- Host Species:
- Mouse
- Isotype:
- IgG
- Antibody Type:
- Monoclonal Antibody
Frequently bought together:
Description
| 抗体名: | Anti-Mouse anti HA-Tag Monoclonal Antibody |
| 抗体コード: | CABE008 |
| 抗体サイズ: | 100 uL |
| 申し込み: | WB IF IP |
| 反応性: | |
| 宿主種: | Mouse |
| 免疫原: | A synthetic peptide corresponding to HA tag. |
| 申し込み: | WB IF IP |
| 推奨希釈: | WB 1:2000 - 1:5000 IF 1:50 - 1:500 IP 1:20 - 1:50 |
| 反応性: | |
| ポジティブサンプル: | 293T |
| 免疫原: | A synthetic peptide corresponding to HA tag. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | YPYD VPDY A |
| 遺伝子ID: | |
| Uniprot: | |
| セルラーロケーション: | |
| 計算された分子量: | |
| 観察された分子量: | Refer to Figures |
 | Western blot analysis of over-expressed HA-tagged protein in 293T cell using HA-tag antibody (CABE008) at different dilution. Each lane was loaded with 2 ug cell lysate. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of 293T cells transfected with HA-Tag fusion protein and untreated 293T cells use Mouse anti HA-Tag mAb (CABE008) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation of over-expressed HA-tagged protein in 293T cells using HA-tag antibody (CABE008). A mock served as negative control and over-expressed 293T cell lysate served as positive control. |
| 同義語: | HA, HA tag, HA-tag |
| バックグラウンド: | Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. |