Anti-MMP9 Antibody (CAB11147)

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SKU:
CAB11147
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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Description

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抗体名:Anti-MMP9 Antibody
抗体コード:CAB11147
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB IHC
反応性:Human, Mouse, Rat
宿主種:Rabbit
免疫原:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human MMP9 (NP_004985.2).
申し込み:WB IHC
推奨希釈:WB 1:500 - 1:2000IHC 1:100 - 1:200
反応性:Human, Mouse, Rat
ポジティブサンプル:Mouse spleen, Rat kidney
免疫原:A synthetic peptide corresponding to a sequence within amino acids 400-500 of human MMP9 (NP_004985.2).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:AHEF GHAL GLDH SSVP EALM YPMY RFTE GPPL HKDD VNGI RHLY GPRP EPEP RPPT TTTP QPTA PPTV CPTG PPTV HPSE RPTA GPTG PPSA GPTG PPTA G
遺伝子ID:4318
Uniprot:P14780
セルラーロケーション:Secreted, extracellular matrix, extracellular space
計算された分子量:78kDa
観察された分子量:92KDa
同義語:MMP9, CLG4B, GELB, MANDP2, MMP-9
バックグラウンド:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
UniProt Protein Function:MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.
UniProt Protein Details:

Protein type:Protease; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.4.24.35

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function:collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity

Biological Process: positive regulation of keratinocyte migration; extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; ossification; macrophage differentiation; positive regulation of apoptosis; ephrin receptor signaling pathway; proteolysis; leukocyte migration; skeletal development; embryo implantation

Disease: Metaphyseal Anadysplasia 2

NCBI Summary:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]
UniProt Code:P14780
NCBI GenInfo Identifier:269849668
NCBI Gene ID:4318
NCBI Accession:P14780.3
UniProt Secondary Accession:P14780,Q3LR70, Q8N725, Q9H4Z1, Q9UCJ9, Q9UCL1, Q9UDK2 B2R7V9,
UniProt Related Accession:P14780
Molecular Weight:78,458 Da
NCBI Full Name:Matrix metalloproteinase-9
NCBI Synonym Full Names:matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
NCBI Official Symbol:MMP9  
NCBI Official Synonym Symbols:GELB; CLG4B; MMP-9; MANDP2  
NCBI Protein Information:matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
UniProt Protein Name:Matrix metalloproteinase-9
UniProt Synonym Protein Names:92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB
Protein Family:Matrix metalloproteinase
UniProt Gene Name:MMP9  
UniProt Entry Name:MMP9_HUMAN
Anti-MMP9 Antibody (CAB11147)Western blot analysis of extracts of various cell lines, using MMP9 antibody (CAB11147) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-MMP9 Antibody (CAB11147)Immunohistochemistry of paraffin-embedded human liver using MMP9 antibody (CAB11147) at dilution of 1:100 (40x lens).
Anti-MMP9 Antibody (CAB11147)Immunohistochemistry of paraffin-embedded mouse spleen using MMP9 antibody (CAB11147) at dilution of 1:100 (40x lens).
Anti-MMP9 Antibody (CAB11147)Immunohistochemistry of paraffin-embedded rat spleen using MMP9 antibody (CAB11147) at dilution of 1:100 (40x lens).
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