Anti-MLST8 Antibody (CAB1059)[KO Validated]
![Anti-MLST8 Antibody (CAB1059)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/21748/21600/anti-mlst8-antibody-cab1059ko-validated__64953__02816.1645804989.jpg?c=1 "Anti-MLST8 Antibody (CAB1059)[KO Validated]")
- SKU:
- CAB1059
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
![Anti-MLST8 Antibody (CAB1059)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/21748/21600/anti-mlst8-antibody-cab1059ko-validated__64953__02816.1645804989.jpg?c=1&imbypass=on "Anti-MLST8 Antibody (CAB1059)[KO Validated]")
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Description
| 抗体名: | Anti-MLST8 Antibody [KO Validated] |
| 抗体コード: | CAB1059 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human MLST8 (NP_001186103.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 293T, HeLa, K-562, 22Rv1, Mouse brain, Mouse kidney, Mouse thymus, Rat brain, Rat testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human MLST8 (NP_001186103.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MNTS PGTV GSDP VILA TAGY DHTV RFWQ AHSG ICTR TVQH QDSQ VNAL EVTP DRSM IAAA GYQH IRMY DLNS NNPN PIIS YDGV NKNI ASVG FHED GRWM YTGG EDCT ARIW DLRS RNLQ CQRI FQVN APIN CVCL HPNQ AELI VGDQ SGAI HIWD LKTD HNEQ LIPE PEVS ITSA HIDP DASY MAAV NSTG NCYV WNLT GGIG DEVT QLIP KTKI PAHT RYAL QCRF SPDS TLLA TCSA DQTC KIWR TSNF SLMT ELSI KSGN PGES SRGW MWGC AFSG DSQY IVTA SSDN LARL WCVE TGEI KREY GGHQ KAVV CLAF NDSV LG |
| 遺伝子ID: | 64223 |
| Uniprot: | Q9BVC4 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 23kDa/28kDa/35kDa |
| 観察された分子量: | 37kDa |
| 同義語: | MLST8, GBL, GbetaL, LST8, POP3, WAT1 |
| バックグラウンド: |
| UniProt Protein Function: | LST8: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient- poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum- induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12- rapamycin. Interacts directly with MTOR and RPTOR. Interacts with RHEB. Broadly expressed, with highest levels in skeletal muscle, heart and kidney. Belongs to the WD repeat LST8 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Autophagy Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm; cytoplasm; cytosol Molecular Function:protein binding Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of actin filament polymerization; nerve growth factor receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway |
| UniProt Code: | Q9BVC4 |
| NCBI GenInfo Identifier: | 74761285 |
| NCBI Gene ID: | 64223 |
| NCBI Accession: | Q9BVC4.1 |
| UniProt Related Accession: | Q9BVC4 |
| Molecular Weight: | |
| NCBI Full Name: | Target of rapamycin complex subunit LST8 |
| NCBI Synonym Full Names: | MTOR associated protein, LST8 homolog |
| NCBI Official Symbol: | MLST8 |
| NCBI Official Synonym Symbols: | GBL; LST8; POP3; WAT1; GbetaL |
| NCBI Protein Information: | target of rapamycin complex subunit LST8 |
| UniProt Protein Name: | Target of rapamycin complex subunit LST8 |
| UniProt Synonym Protein Names: | G protein beta subunit-like; Gable; Protein GbetaL; Mammalian lethal with SEC13 protein 8; mLST8 |
| UniProt Gene Name: | MLST8 |
| UniProt Entry Name: | LST8_HUMAN |
![Anti-MLST8 Antibody [KO Validated] (CAB1059)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/j/298/A1059_1__27408.jpg) | Western blot analysis of extracts of various cell lines, using MLST8 antibody (CAB1059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
![Anti-MLST8 Antibody [KO Validated] (CAB1059)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/c/402/A1059_2__10560.jpg) | Western blot analysis of extracts from normal (control) and MLST8 knockout (KO) HeLa cells, using MLST8 antibody (CAB1059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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