Anti-MLPH Antibody (CAB6656)
- SKU:
- CAB6656
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MLPH Antibody |
| 抗体コード: | CAB6656 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 301-600 of human MLPH (NP_077006.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Mouse stomach |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 301-600 of human MLPH (NP_077006.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EQLP LQYL ADVD TSDE ESIR AHVM ASHH SKRR GRAS SESQ IFEL NKHI SAVE CLLT YLEN TVVP PLAK GLGA GVRT EADV EEEA LRRK LEEL TSNV SDQE TSSE EEEA KDEK AEPN RDKS VGPL PQAD PEVG TAAH QTNR QEKS PQDP GDPV QYNR TTDE ELSE LEDR VAVT ASEV QQAE SEVS DIES RIAA LRAA GLTV KPSG KPRR KSNL PIFL PRVA GKLG KRPE DPNA DPSS EAKA MAVP YLLR RKFS NSLK SQGK DDDS FDRK SVYR GSLT QRNP NARK GMAS HTFA KPVV AHQS |
| 遺伝子ID: | 79083 |
| Uniprot: | Q9BV36 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 50kDa/52kDa/60kDa/62kDa/65kDa |
| 観察された分子量: | 75kDa |
| 同義語: | MLPH, SLAC2-A |
| バックグラウンド: | This gene encodes a member of the exophilin subfamily of Rab effector proteins. The protein forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. A similar protein complex in mouse functions to tether pigment-producing organelles called melanosomes to the actin cytoskeleton in melanocytes, and is required for visible pigmentation in the hair and skin. A mutation in this gene results in Griscelli syndrome type 3, which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | MLPH: Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3). GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 2q37.3 Cellular Component: cortical actin cytoskeleton; perinuclear region of cytoplasm; dendrite; stress fiber; microtubule organizing center Molecular Function:microtubule plus-end binding; metal ion binding; myosin V binding; actin binding; Rab GTPase binding Biological Process: melanocyte differentiation; melanosome localization; protein targeting Disease: Griscelli Syndrome, Type 3 |
| NCBI Summary: | This gene encodes a member of the exophilin subfamily of Rab effector proteins. The protein forms a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. A similar protein complex in mouse functions to tether pigment-producing organelles called melanosomes to the actin cytoskeleton in melanocytes, and is required for visible pigmentation in the hair and skin. A mutation in this gene results in Griscelli syndrome type 3, which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9BV36 |
| NCBI GenInfo Identifier: | 32129730 |
| NCBI Gene ID: | 79083 |
| NCBI Accession: | Q9BV36.1 |
| UniProt Secondary Accession: | Q9BV36,Q9HA71, B3KSS2, B4DKW7, G5E9G5, |
| UniProt Related Accession: | Q9BV36 |
| Molecular Weight: | 600 |
| NCBI Full Name: | Melanophilin |
| NCBI Synonym Full Names: | melanophilin |
| NCBI Official Symbol: | MLPH |
| NCBI Official Synonym Symbols: | SLAC2-A |
| NCBI Protein Information: | melanophilin; exophilin-3; synaptotagmin-like protein 2a; slp homolog lacking C2 domains a |
| UniProt Protein Name: | Melanophilin |
| UniProt Synonym Protein Names: | Exophilin-3; Slp homolog lacking C2 domains a; SlaC2-a; Synaptotagmin-like protein 2a |
| Protein Family: | Melanophilin |
| UniProt Gene Name: | MLPH |
| UniProt Entry Name: | MELPH_HUMAN |
 | Western blot analysis of extracts of Mouse stomach, using MLPH antibody (CAB6656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunofluorescence analysis of NIH-3T3 cells using MLPH antibody (CAB6656) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using MLPH antibody (CAB6656) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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