Anti-MICAL1 Antibody (CAB15505)

(No reviews yet) Write a Review
お見積り依頼
SKU:
CAB15505
Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€0.00
Frequently bought together:

Description

system_update_altデータシートsystem_update_alt安全性データシート
抗体名:Anti-MICAL1 Antibody
抗体コード:CAB15505
抗体サイズ:20uL, 50uL, 100uL
申し込み:WB
反応性:Human, Mouse
宿主種:Rabbit
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 718-1067 of human MICAL1 (NP_073602.3).
申し込み:WB
推奨希釈:WB 1:200 - 1:2000
反応性:Human, Mouse
ポジティブサンプル:A-549, HT-29, Raji
免疫原:Recombinant fusion protein containing a sequence corresponding to amino acids 718-1067 of human MICAL1 (NP_073602.3).
精製方法:Affinity purification
ストレージバッファ:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
アイソタイプ:IgG
順序:HRSC FRCH TCEA TLWP GGYE QHPG DGHF YCLQ HLPQ TDHK AEGS DRGP ESPE LPTP SENS MPPG LSTP TASQ EGAG PVPD PSQP TRRQ IRLS SPER QRLS SLNL TPDP EMEP PPKP PRSC SALA RHAL ESSF VGWG LPVQ SPQA LVAM EKEE KESP FSSE EEEE DVPL DSDV EQAL QTFA KTSG TMNN YPTW RRTL LRRA KEEE MKRF CKAQ TIQR RLNE IEAA LREL EAEG VKLE LALR RQSS SPEQ QKKL WVGQ LLQL VDKK NSLV AEEA ELMI TVQE LNLE EKQW QLDQ ELRG YMNR EENL KTAA DRQA EDQV LRKL VDLV NQRD ALIR FQEE RRLS ELAL GTGA QG
遺伝子ID:64780
Uniprot:Q8TDZ2
セルラーロケーション:Cytoplasm, cytoskeleton
計算された分子量:37kDa/108kDa/117kDa/119kDa
観察された分子量:132kDa
同義語:MICAL1, MICAL, MICAL-1, NICAL
バックグラウンド:This gene encodes an enzyme that oxidizes methionine residues on actin, thereby promoting depolymerization of actin filaments. This protein interacts with and regulates signalling by NEDD9/CAS-L (neural precursor cell expressed, developmentally down-regulated 9). Alternative splicing results in multiple transcript variants.
UniProt Protein Function:MICAL1: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (Probable). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4. Belongs to the Mical family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold; EC 1.14.13.-

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; intermediate filament

Molecular Function:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen; protein binding; zinc ion binding; actin binding; SH3 domain binding; Rab GTPase binding

Biological Process: actin filament depolymerization; negative regulation of protein amino acid phosphorylation; cytoskeleton organization and biogenesis; negative regulation of caspase activity; signal transduction; sulfur oxidation

UniProt Code:Q8TDZ2
NCBI GenInfo Identifier:45593495
NCBI Gene ID:
NCBI Accession:Q8TDZ2.2
Molecular Weight:~ 132kDa
NCBI Full Name:[F-actin]-monooxygenase MICAL1
UniProt Protein Name:Protein-methionine sulfoxide oxidase MICAL1
UniProt Synonym Protein Names:Molecule interacting with CasL protein 1; MICAL-1; NEDD9-interacting protein with calponin homology and LIM domains
Protein Family:Protein-methionine sulfoxide oxidase
UniProt Gene Name:MICAL1  
UniProt Entry Name:MICA1_HUMAN
Anti-MICAL1 Antibody (CAB15505)Western blot analysis of extracts of various cell lines, using MICAL1 antibody (CAB15505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
View AllClose

View AllClose