Anti-MGMT Antibody (CAB0052)
- SKU:
- CAB0052
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-MGMT Antibody |
| 抗体コード: | CAB0052 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (XP_005252739.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Rat |
| ポジティブサンプル: | Jurkat |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (XP_005252739.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MDKD CEMK RTTL DSPL GKLE LSGC EQGL HEIK LLGK GTSA ADAV EVPA PAAV LGGP EPLM QCTA WLNA YFHQ PEAI EEFP VPAL HHPV FQQE SFTR QVLW KLLK VVKF GEVI SYQQ LAAL AGNP KAAR AVGG AMRG NPVP ILIP CHRV VCSS GAVG NYSG GLAV KEWL LAHE GHRL GKPG LGGS SGLA GAWL KGAG ATSG SPPA GRN |
| 遺伝子ID: | 4255 |
| Uniprot: | P16455 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 21kDa |
| 観察された分子量: | 22kDa |
| 同義語: | MGMT |
| バックグラウンド: | Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. |
| UniProt Protein Function: | MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family. |
| UniProt Protein Details: | Protein type:EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage; Apoptosis Chromosomal Location of Human Ortholog: 10q26 Cellular Component: nucleoplasm; membrane; nucleus Molecular Function:methyltransferase activity; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation |
| UniProt Code: | P16455 |
| NCBI GenInfo Identifier: | 127069 |
| NCBI Gene ID: | 4255 |
| NCBI Accession: | P16455.1 |
| UniProt Secondary Accession: | P16455,Q5VY78, |
| UniProt Related Accession: | P16455 |
| Molecular Weight: | 21,646 Da |
| NCBI Full Name: | Methylated-DNA--protein-cysteine methyltransferase |
| NCBI Synonym Full Names: | O-6-methylguanine-DNA methyltransferase |
| NCBI Official Symbol: | MGMT |
| NCBI Protein Information: | methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase |
| UniProt Protein Name: | Methylated-DNA--protein-cysteine methyltransferase |
| UniProt Synonym Protein Names: | 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase |
| Protein Family: | Probable methylated-DNA--protein-cysteine methyltransferase |
| UniProt Gene Name: | MGMT |
| UniProt Entry Name: | MGMT_HUMAN |
 | Western blot analysis of extracts of Jurkat cells, using MGMT antibody (CAB0052) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s. |
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