Anti-METTL3 Antibody [KO Validated] (CAB19079)
![Anti-METTL3 Antibody [KO Validated] (CAB19079)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/26338/26186/anti-mettl3-antibody-ko-validated-cab19079__99104__41046.1645806461.jpg?c=1 "Anti-METTL3 Antibody [KO Validated] (CAB19079)")
- SKU:
- CAB19079
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
![Anti-METTL3 Antibody [KO Validated] (CAB19079)](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/26338/26186/anti-mettl3-antibody-ko-validated-cab19079__99104__41046.1645806461.jpg?c=1&imbypass=on "Anti-METTL3 Antibody [KO Validated] (CAB19079)")
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Description
| 抗体名: | Anti-METTL3 Antibody [KO Validated] |
| 抗体コード: | CAB19079 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | A synthesized peptide derived from human METTL3 |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HeLa, Mouse brain, Mouse testis, 293T |
| 免疫原: | A synthesized peptide derived from human METTL3 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 56339 |
| Uniprot: | Q86U44 |
| セルラーロケーション: | Nucleus speckle |
| 計算された分子量: | 70kDa |
| 観察された分子量: | 80kDa |
![Anti-METTL3 Antibody [KO Validated] (CAB19079)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/a/000/A19079_1__21260.jpg) | Western blot analysis of extracts from normal (control) and METTL3 knockout (KO) 293T cells, using METTL3 antibody (CAB19079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
![Anti-METTL3 Antibody [KO Validated] (CAB19079)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/o/409/A19079_2__72926.jpg) | Western blot analysis of extracts of various cell lines, using METTL3 antibody (CAB19079) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min. |
![Anti-METTL3 Antibody [KO Validated] (CAB19079)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/c/575/A19079_3__34305.jpg) | Immunofluorescence analysis of HeLa cells using METTL3 Rabbit mAb (CAB19079) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
| 同義語: | IME4, M6A, MT-A70, Spo8, hMETTL3, METTL3 |
| バックグラウンド: | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008] |
| UniProt Protein Function: | METTL3: N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation. Belongs to the MT-A70-like family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Methyltransferase; EC 2.1.1.62; RNA processing Chromosomal Location of Human Ortholog: 14q11.1 Cellular Component: nucleoplasm; nuclear speck; nucleus Molecular Function:mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity; RNA binding Biological Process: circadian rhythm; stem cell maintenance; RNA methylation; gene expression; mRNA processing |
| NCBI Summary: | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q86U44 |
| NCBI GenInfo Identifier: | 21361827 |
| NCBI Gene ID: | 56339 |
| NCBI Accession: | NP_062826.2 |
| UniProt Secondary Accession: | Q86U44,O14736, Q86V05, Q9HB32, |
| UniProt Related Accession: | Q86U44 |
| Molecular Weight: | 25,479 Da |
| NCBI Full Name: | N6-adenosine-methyltransferase 70 kDa subunit |
| NCBI Synonym Full Names: | methyltransferase like 3 |
| NCBI Official Symbol: | METTL3 |
| NCBI Official Synonym Symbols: | M6A; IME4; Spo8; MT-A70 |
| NCBI Protein Information: | N6-adenosine-methyltransferase 70 kDa subunit; mRNA m(6)A methyltransferase; methyltransferase-like protein 3; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase |
| UniProt Protein Name: | N6-adenosine-methyltransferase 70 kDa subunit |
| UniProt Synonym Protein Names: | Methyltransferase-like protein 3 |
| Protein Family: | N6-adenosine-methyltransferase |
| UniProt Gene Name: | METTL3 |
| UniProt Entry Name: | MTA70_HUMAN |