Anti-MED7 Antibody (CAB16088)
- SKU:
- CAB16088
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-MED7 Antibody |
| 抗体コード: | CAB16088 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human MED7 (NP_004261.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | 293T, NIH/3T3, Mouse testis |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human MED7 (NP_004261.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGEP QQVS ALPP PPMQ YIKE YTDE NIQE GLAP KPPP PIKD SYMM FGNQ FQCD DLII RPLE SQGI ERLH PMQF DHKK ELRK LNMS ILIN FLDL LDIL IRSP GSIK REEK LEDL KLLF VHVH HLIN EYRP HQAR ETLR VMME VQKR QRLE TAER FQKH LERV IEMI QNCL ASLP DDLP HSEA GMRV KTEP MDAD DSNN CTGQ NEHQ RENS GHRR DQII EKDA ALCV LIDE MNER P |
| 遺伝子ID: | 9443 |
| Uniprot: | O43513 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 27kDa |
| 観察された分子量: | 27kDa |
| 同義語: | MED7, ARC34, CRSP33, CRSP9 |
| バックグラウンド: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | MED7: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 7 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 5q33.3 Cellular Component: nucleoplasm; Srb-mediator complex; transcription factor complex Molecular Function:protein binding; transcription coactivator activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter |
| NCBI Summary: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43513 |
| NCBI GenInfo Identifier: | 29337159 |
| NCBI Gene ID: | 9443 |
| NCBI Accession: | O43513.1 |
| UniProt Related Accession: | O43513 |
| Molecular Weight: | 27,245 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 7 |
| NCBI Synonym Full Names: | mediator complex subunit 7 |
| NCBI Official Symbol: | MED7 |
| NCBI Official Synonym Symbols: | ARC34; CRSP9; CRSP33 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 7 |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 7 |
| UniProt Synonym Protein Names: | Activator-recruited cofactor 34 kDa component; ARC34; Cofactor required for Sp1 transcriptional activation subunit 9; CRSP complex subunit 9; Mediator complex subunit 7; RNA polymerase transcriptional regulation mediator subunit 7 homolog; Transcriptional coactivator CRSP33 |
| Protein Family: | Putative mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED7 |
| UniProt Entry Name: | MED7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MED7 antibody (CAB16088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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