Anti-MED31 Antibody (CAB15831)
- SKU:
- CAB15831
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| 抗体名: | Anti-MED31 Antibody |
| 抗体コード: | CAB15831 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human MED31 (NP_057144.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, Mouse brain, Mouse liver, Mouse testis, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-131 of human MED31 (NP_057144.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAA VAME TDDA GNRL RFQL ELEF VQCL ANPN YLNF LAQR GYFK DKAF VNYL KYLL YWKD PEYA KYLK YPQC LHML ELLQ YEHF RKEL VNAQ CAKF IDEQ QILH WQHY SRKR MRLQ QALA EQQQ QNNT SGK |
| 遺伝子ID: | 51003 |
| Uniprot: | Q9Y3C7 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 15kDa |
| 観察された分子量: | 16kDa |
| 同義語: | MED31, 3110004H13Rik, CGI-125, Soh1 |
| バックグラウンド: |
| UniProt Protein Function: | MED31: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 31 family. |
| UniProt Protein Details: | Protein type:Transcription regulation Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: nucleoplasm; Srb-mediator complex Molecular Function:protein binding; protein complex binding Biological Process: limb development; regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; negative regulation of fibroblast proliferation; protein complex assembly; gene expression |
| UniProt Code: | Q9Y3C7 |
| NCBI GenInfo Identifier: | 7705592 |
| NCBI Gene ID: | 51003 |
| NCBI Accession: | NP_057144.1 |
| UniProt Secondary Accession: | Q9Y3C7,B2R4L9, |
| UniProt Related Accession: | Q9Y3C7 |
| Molecular Weight: | 15,805 Da |
| NCBI Full Name: | mediator of RNA polymerase II transcription subunit 31 |
| NCBI Synonym Full Names: | mediator complex subunit 31 |
| NCBI Official Symbol: | MED31 |
| NCBI Official Synonym Symbols: | Soh1; CGI-125; 3110004H13Rik |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 31; hSOH1; mediator complex subunit SOH1; mediator of RNA polymerase II transcription, subunit 31 homolog |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 31 |
| UniProt Synonym Protein Names: | Mediator complex subunit 31; Mediator complex subunit SOH1; hSOH1 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED31 |
| UniProt Entry Name: | MED31_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MED31 antibody (CAB15831) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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