Anti-MED12 Antibody (CAB18302)
- SKU:
- CAB18302
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| 抗体名: | Anti-MED12 Antibody |
| 抗体コード: | CAB18302 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human MED12. |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: |
| 免疫原: | Recombinant protein of human MED12. |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 9968 |
| Uniprot: | Q93074 |
| セルラーロケーション: | |
| 計算された分子量: | 243kDa |
| 観察された分子量: | Refer to figures |
| 同義語: | MED12, ARC240, CAGH45, FGS1, HOPA, MED12S, OHDOX, OKS, OPA1, TNRC11, TRAP230 |
| バックグラウンド: | The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. |
| UniProt Protein Function: | MED12: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: Xq13 Cellular Component: centrosome; membrane; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; ubiquitin ligase complex Molecular Function:beta-catenin binding; chromatin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein C-terminus binding; protein domain specific binding; receptor activity; thyroid hormone receptor binding; transcription coactivator activity; transcription cofactor activity; vitamin D receptor binding Biological Process: androgen receptor signaling pathway; embryonic neurocranium morphogenesis; endoderm development; heart development; negative regulation of Wnt receptor signaling pathway; neural tube closure; oligodendrocyte development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein ubiquitination; Schwann cell development; spinal cord development; stem cell maintenance; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway Disease: Lujan-fryns Syndrome; Ohdo Syndrome, X-linked; Opitz-kaveggia Syndrome |
| NCBI Summary: | The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009] |
| UniProt Code: | Q93074 |
| NCBI GenInfo Identifier: | 110347429 |
| NCBI Gene ID: | 9968 |
| NCBI Accession: | NP_005111.2 |
| UniProt Secondary Accession: | Q93074,O15410, O75557, Q9UHV6, Q9UND7, |
| UniProt Related Accession: | Q93074 |
| Molecular Weight: | 242,953 Da |
| NCBI Full Name: | mediator of RNA polymerase II transcription subunit 12 |
| NCBI Synonym Full Names: | mediator complex subunit 12 |
| NCBI Official Symbol: | MED12 |
| NCBI Official Synonym Symbols: | OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230 |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 12 |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 12 |
| UniProt Synonym Protein Names: | Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; Mediator complex subunit 12; OPA-containing protein; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Trinucleotide repeat-containing gene 11 protein |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED12 |
| UniProt Entry Name: | MED12_HUMAN |