Anti-ME1 Antibody (CAB3956)
- SKU:
- CAB3956
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-ME1 Antibody |
| 抗体コード: | CAB3956 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ME1 (NP_002386.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:1000 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | LO2, MCF7, Mouse heart, Mouse brain, Mouse liver, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ME1 (NP_002386.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEPE APRR RHTH QRGY LLTR NPHL NKDL AFTL EERQ QLNI HGLL PPSF NSQE IQVL RVVK NFEH LNSD FDRY LLLM DLQD RNEK LFYR VLTS DIEK FMPI VYTP TVGL ACQQ YSLV FRKP RGLF ITIH DRGH IASV LNAW PEDV IKAI VVTD GERI LGLG DLGC NGMG IPVG KLAL YTAC GGMN PQEC LPVI LDVG TENE ELLK DPLY IGLR QRRV RGSE YDDF LDEF MEAV SSKY GMNC LIQF EDFA NVNA FRLL NKYR NQYC TFND DIQG TASV AVAG LLAA LRIT KNKL SDQT ILFQ |
| 遺伝子ID: | 4199 |
| Uniprot: | P48163 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 55kDa/64kDa |
| 観察された分子量: | 64kDa |
| 同義語: | ME1, HUMNDME, MES |
| バックグラウンド: | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. |
| UniProt Protein Function: | ME1: a ubiquitous lipogenic enzyme of the malic enzymes family. Converts (S)-malate + NADP+ ?? pyruvate + CO2 + NADPH. Levels of ME1 and ATP-citrate lyase mRNA, another lipogenic enzyme, are coordinately increased 5-7 fold during the differentiation of 3T3-L1 preadipocytes into adipocytes. Up-regulated by short-term dietary restriction in mice. The cycling of pyruvate by ME1 generates cytosolic NADPH, whereas mitochondrial ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting. |
| UniProt Protein Details: | Protein type:Oxidoreductase; EC 1.1.1.40; Carbohydrate Metabolism - pyruvate Chromosomal Location of Human Ortholog: 6q12 Cellular Component: mitochondrion; cytosol Molecular Function:malate dehydrogenase (decarboxylating) activity; malic enzyme activity; electron carrier activity; manganese ion binding; malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; ADP binding; NADP binding; NAD binding; oxaloacetate decarboxylase activity Biological Process: malate metabolic process; carbohydrate metabolic process; response to hormone stimulus; NADP biosynthetic process; cellular lipid metabolic process; response to carbohydrate stimulus; protein tetramerization |
| NCBI Summary: | This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P48163 |
| NCBI GenInfo Identifier: | 1346484 |
| NCBI Gene ID: | 4199 |
| NCBI Accession: | P48163.1 |
| UniProt Secondary Accession: | P48163,Q16797, Q16855, Q53F72, Q5VWA2, Q9BWX8, Q9H1W3 Q9UIY4, B4DZ70, |
| UniProt Related Accession: | P48163 |
| Molecular Weight: | 572 |
| NCBI Full Name: | NADP-dependent malic enzyme |
| NCBI Synonym Full Names: | malic enzyme 1, NADP(+)-dependent, cytosolic |
| NCBI Official Symbol: | ME1 |
| NCBI Official Synonym Symbols: | MES; HUMNDME |
| NCBI Protein Information: | NADP-dependent malic enzyme; NADP-ME; malate dehydrogenase; malic enzyme 1, soluble; Malic enzyme, cytoplasmic; pyruvic-malic carboxylase |
| UniProt Protein Name: | NADP-dependent malic enzyme |
| UniProt Synonym Protein Names: | Malic enzyme 1 |
| Protein Family: | NADP-dependent malic enzyme |
| UniProt Gene Name: | ME1 |
| UniProt Entry Name: | MAOX_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ME1 antibody (CAB3956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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