Anti-MCPH1 Antibody (CAB5472)
- SKU:
- CAB5472
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MCPH1 Antibody |
| 抗体コード: | CAB5472 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human MCPH1 (NP_001166046.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | A-549, HeLa, 293T, 3T3L1, SH-SY5Y, Mouse testis, Mouse brain, Mouse liver, Mouse kidney |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 263-562 of human MCPH1 (NP_001166046.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VTPD QKQA AGMS QETF EEKY RLSP TLSS TKGH LLIH SRPR SSSV KRKR VSHG SHSP PKEK CKRK RSTR RSIM PRLQ LCRS EDRL QHVA GPAL EALS CGES SYDD YFSP DNLK ERYS ENLP PESQ LPSS PAQL SCRS LSKK ERTS IFEM SDFS CVGK KTRT VDIT NFTA KTIS SPRK TGNG EGRA TSSC VTSA PEEA LRCC RQAG KEDA CPEG NGFS YTIE DPAL PKGH DDDL TPLE GSLE EMKE AVGL KSTQ NKGT TSKI SNSS EGEA QSEH EPCF IVDC NMET STEE KENL PGGY SGSM |
| 遺伝子ID: | 79648 |
| Uniprot: | Q8NEM0 |
| セルラーロケーション: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center |
| 計算された分子量: | 62kDa/67kDa/92kDa |
| 観察された分子量: | 60kDa |
| 同義語: | MCPH1, BRIT1, MCT |
| バックグラウンド: | This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | MCPH1: a DNA damage responsive protein and transcriptional repressor of human telomere reverse transcriptase. An early responder to DNA damage. Required for double strand break repair via homologous recombination and nonhomologous end joining. Promotes chromatin relaxation by recruiting and maintaining the chromatin remodeling SWI-SNF complex at sites of DNA double strand breakage. Participates in the ATR/Chk1-mediated DNA checkpoint in humans and transcriptionally regulates BRCA1 and Chk1. Plays a role in neurogenesis and regulation of the size of the cerebral cortex. Mutations of MCPH1 can cause microcephaly in humans and appears to play an evolutionarily conserved role in brain development. Alterations have been identified in breast, ovarian and prostate cancer. Appears to coordinate S-M transitions in fly embryos. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 8p23.1 Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center Molecular Function:identical protein binding; protein binding Biological Process: cerebral cortex development; mitotic cell cycle Disease: Microcephaly 1, Primary, Autosomal Recessive |
| NCBI Summary: | This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010] |
| UniProt Code: | Q8NEM0 |
| NCBI GenInfo Identifier: | 296439305 |
| NCBI Gene ID: | 79648 |
| NCBI Accession: | Q8NEM0.3 |
| UniProt Secondary Accession: | Q8NEM0,Q66GU1, Q9H9C7, B4DWW2, E9PGU5, E9PH63, |
| UniProt Related Accession: | Q8NEM0 |
| Molecular Weight: | 835 |
| NCBI Full Name: | Microcephalin |
| NCBI Synonym Full Names: | microcephalin 1 |
| NCBI Official Symbol: | MCPH1 |
| NCBI Official Synonym Symbols: | MCT; BRIT1 |
| NCBI Protein Information: | microcephalin; BRCT-repeat inhibitor of TERT expression 1 |
| UniProt Protein Name: | Microcephalin |
| Protein Family: | Microcephalin |
| UniProt Gene Name: | MCPH1 |
| UniProt Entry Name: | MCPH1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MCPH1 antibody (CAB5472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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