Anti-MCM8 Antibody (CAB12922)
- SKU:
- CAB12922
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-MCM8 Antibody |
| 抗体コード: | CAB12922 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 591-840 of human MCM8 (NP_115874.3). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | LO2, HeLa, A-431 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 591-840 of human MCM8 (NP_115874.3). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | FILL DTPN EHHD HLLS EHVI AIRA GKQR TISS ATVA RMNS QDSN TSVL EVVS EKPL SERL KVVP GETI DPIP HQLL RKYI GYAR QYVY PRLS TEAA RVLQ DFYL ELRK QSQR LNSS PITT RQLE SLIR LTEA RARL ELRE EATK EDAE DIVE IMKY SMLG TYSD EFGN LDFE RSQH GSGM SNRS TAKR FISA LNNV AERT YNNI FQFH QLRQ IAKE LNIQ VADF ENFI GSLN DQGY LLKK GPKV YQLQ TM |
| 遺伝子ID: | 84515 |
| Uniprot: | Q9UJA3 |
| セルラーロケーション: | Nucleus |
| 計算された分子量: | 88kDa/91kDa/93kDa/97kDa |
| 観察された分子量: | 110kDa |
| 同義語: | MCM8, C20orf154, POF10, dJ967N21.5 |
| バックグラウンド: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. |
| UniProt Protein Function: | MCM8: Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8- MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle. Belongs to the MCM family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; EC 3.6.4.12 Chromosomal Location of Human Ortholog: 20p12.3 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; DNA binding; helicase activity; ATP binding Biological Process: male gamete generation; female gamete generation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; response to DNA damage stimulus; double-strand break repair via homologous recombination; G1/S transition of mitotic cell cycle Disease: Premature Ovarian Failure 10 |
| NCBI Summary: | The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9UJA3 |
| NCBI GenInfo Identifier: | 27805609 |
| NCBI Gene ID: | 84515 |
| NCBI Accession: | Q9UJA3.2 |
| UniProt Secondary Accession: | Q9UJA3,Q495R4, Q495R6, Q495R7, Q86US4, Q969I5, B2RBG7 D3DW08, E7EQU7, |
| UniProt Related Accession: | Q9UJA3 |
| Molecular Weight: | 840 |
| NCBI Full Name: | DNA helicase MCM8 |
| NCBI Synonym Full Names: | minichromosome maintenance complex component 8 |
| NCBI Official Symbol: | MCM8 |
| NCBI Official Synonym Symbols: | C20orf154; dJ967N21.5 |
| NCBI Protein Information: | DNA helicase MCM8; REC homolog; DNA replication licensing factor MCM8; MCM8 minichromosome maintenance deficient 8 |
| UniProt Protein Name: | DNA helicase MCM8 |
| UniProt Synonym Protein Names: | Minichromosome maintenance 8 |
| Protein Family: | DNA helicase |
| UniProt Gene Name: | MCM8 |
| UniProt Entry Name: | MCM8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MCM8 antibody (CAB12922) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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