Anti-MCF2 Antibody (CAB12839)
- SKU:
- CAB12839
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MCF2 Antibody |
| 抗体コード: | CAB12839 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 872-1001 of human MCF2 (NP_001165347.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HT-29 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 872-1001 of human MCF2 (NP_001165347.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | VKMT WLKE IRNI LLKQ QELL TVKK RKQQ DQLT ERDK FQIS LQQN DEKQ QGAF ISTE ETEL EHTS TVVE VCEA IASV QAEA NTVW TEAS QSAE ISEE PAEW SSNY FYPT YDEN EEEN RPLM RPVS EMAL LY |
| 遺伝子ID: | 4168 |
| Uniprot: | P10911 |
| セルラーロケーション: | Cytoplasm, Membrane |
| 計算された分子量: | 95kDa/100kDa/107kDa/109kDa/114kDa/116kDa |
| 観察された分子量: | 108kDa |
| 同義語: | MCF2, ARHGEF21, DBL |
| バックグラウンド: | The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity. |
| UniProt Protein Function: | MCF2: Guanine nucleotide exchange factor (GEF) that modulates the Rho family of GTPases. Promotes the conversion of some member of the Rho family GTPase from the GDP-bound to the GTP-bound form. Isoform 1 exhibits no activity toward RHOA, RAC1 or CDC42. Isoform 2 exhibits decreased GEF activity toward CDC42. Isoform 3 exhibits a weak but significant activity toward RAC1 and CDC42. Isoform 4 exhibits significant activity toward RHOA and CDC42. The truncated DBL oncogene is active toward RHOA, RAC1 and CDC42. Interacts with an array of inositol phospholipids such as phosphatidylinositol 3-phosphate (PI3P), phosphatidylinositol 4- phosphate (PI4P) and phosphatidylinositol 5-phosphate (PI5P). May interact with CCPG1. Isoform 1 is expressed only in brain. Isoform 3 is expressed in heart, kidney, spleen, liver and testis. Isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach and peripheral blood. The protein is detectable in brain, heart, kidney, intestine, muscle, lung and testis. Belongs to the MCF2 family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis; Cell development/differentiation; GEF; GEF, Rac/Rho; Motility/polarity/chemotaxis; Oncoprotein Chromosomal Location of Human Ortholog: Xq27.1 Cellular Component: cytoskeleton; cytosol Molecular Function:guanyl-nucleotide exchange factor activity; protein binding; Rho guanyl-nucleotide exchange factor activity Biological Process: positive regulation of apoptosis; regulation of small GTPase mediated signal transduction |
| NCBI Summary: | The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010] |
| UniProt Code: | P10911 |
| NCBI GenInfo Identifier: | 92087039 |
| NCBI Gene ID: | 4168 |
| NCBI Accession: | P10911.3 |
| UniProt Secondary Accession: | P10911,P14919, Q5JYJ2, Q5JYJ3, Q5JYJ4, Q8IUF3, Q8IUF4 B7Z3Y5, B7Z869, B7ZAV1, E9PH77, F5H091, |
| UniProt Related Accession: | P10911 |
| Molecular Weight: | 108kDa |
| NCBI Full Name: | Proto-oncogene DBL |
| NCBI Synonym Full Names: | MCF.2 cell line derived transforming sequence |
| NCBI Official Symbol: | MCF2 |
| NCBI Official Synonym Symbols: | DBL; ARHGEF21 |
| NCBI Protein Information: | proto-oncogene DBL |
| UniProt Protein Name: | Proto-oncogene DBL |
| UniProt Synonym Protein Names: | Proto-oncogene MCF-2 |
| Protein Family: | Proto-oncogene |
| UniProt Gene Name: | MCF2 |
 | Western blot analysis of extracts of HT-29 cells, using MCF2 antibody (CAB12839) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
View AllClose