Anti-MARCH8 Antibody (CAB12962)
- SKU:
- CAB12962
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Frequently bought together:
Description
| 抗体名: | Anti-MARCH8 Antibody |
| 抗体コード: | CAB12962 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 232-291 of human MARCH8 (NP_659458.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | 22Rv1, U-87MG, Mouse testis, Rat testis, Rat liver |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 232-291 of human MARCH8 (NP_659458.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | YNRV IYVQ NCPE TSKK NIFE KSPL TEPN FENK HGYG ICHS DTNS SCCT EPED TGAE IIHV |
| 遺伝子ID: | 220972 |
| Uniprot: | Q5T0T0 |
| セルラーロケーション: | Cytoplasmic vesicle membrane, Early endosome membrane, Lysosome membrane, Multi-pass membrane protein |
| 計算された分子量: | 32kDa/63kDa |
| 観察された分子量: | 33kDa |
| 同義語: | MARCH8, CMIR, MARCH-VIII, MIR, RNF178, c-MIR |
| バックグラウンド: |
| UniProt Protein Function: | MARCH8: E3 ubiquitin-protein ligase that mediates ubiquitination of CD86 and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. May also promote ubiquitination and endocytosis of TFRC and FAS. |
| UniProt Protein Details: | Protein type:EC 6.3.2.-; EC 6.3.2.19; Ligase; Membrane protein, integral; Membrane protein, multi-pass; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 10q11.21-q11.22 Cellular Component: endosome; lysosome Molecular Function:ubiquitin-protein ligase activity Biological Process: protein polyubiquitination |
| NCBI Summary: | MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010] |
| UniProt Code: | Q5T0T0 |
| NCBI GenInfo Identifier: | 74744352 |
| NCBI Gene ID: | 220972 |
| NCBI Accession: | Q5T0T0.1 |
| UniProt Secondary Accession: | Q5T0T0,Q5T0S8, Q8TC72, B2R8E7, H0Y7C6, |
| UniProt Related Accession: | Q5T0T0 |
| Molecular Weight: | 33kDa |
| NCBI Full Name: | E3 ubiquitin-protein ligase MARCH8 |
| NCBI Synonym Full Names: | membrane associated ring-CH-type finger 8 |
| NCBI Official Symbol: | MARCH8 |
| NCBI Official Synonym Symbols: | MIR; CMIR; c-MIR; RNF178; MARCH-VIII |
| NCBI Protein Information: | E3 ubiquitin-protein ligase MARCH8 |
| UniProt Protein Name: | E3 ubiquitin-protein ligase MARCH8 |
| UniProt Synonym Protein Names: | Cellular modulator of immune recognition; c-MIR; Membrane-associated RING finger protein 8; Membrane-associated RING-CH protein VIII; MARCH-VIII; RING finger protein 178; RING-type E3 ubiquitin transferase MARCH8Curated |
| UniProt Gene Name: | MARCH8 |
 | Western blot analysis of extracts of various cell lines, using MARCH8 antibody (CAB12962) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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