Anti-MAP4K4 Antibody (CAB11706)
- SKU:
- CAB11706
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MAP4K4 Antibody |
| 抗体コード: | CAB11706 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-650 of human MAP4K4 (NP_663719.2). |
| 申し込み: | WB IHC |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| 反応性: | Human |
| ポジティブサンプル: | U-87MG, HeLa |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 480-650 of human MAP4K4 (NP_663719.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | EVLQ QQLL QEQA MLLH DHRR PHPQ HSQQ PPPP QQER SKPS FHAP EPKA HYEP ADRA REVE DRFR KTNH SSPE AQSK QTGR VLEP PVPS RSES FSNG NSES VHPA LQRP AEPQ VPVR TTSR SPVL SRRD SPLQ GSGQ QNSQ AGQR NSTS SIEP RLLW ERVE KLVP RPGS GSS |
| 遺伝子ID: | 9448 |
| Uniprot: | O95819 |
| セルラーロケーション: | Cytoplasm |
| 計算された分子量: | 133kDa/138kDa/141kDa/142kDa/145kDa/150kDa |
| 観察された分子量: | 142kDa |
| 同義語: | MAP4K4, FLH21957, HEL-S-31, HGK, MEKKK4, NIK |
| バックグラウンド: | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. |
| UniProt Protein Function: | HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protein kinase, STE; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MSN subfamily Chromosomal Location of Human Ortholog: 2q11.2-q12 Cellular Component: cytoplasm Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: microvillus biogenesis; regulation of catalytic activity; protein amino acid phosphorylation; regulation of JNK cascade |
| NCBI Summary: | The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O95819 |
| NCBI GenInfo Identifier: | 29427585 |
| NCBI Gene ID: | 9448 |
| NCBI Accession: | O95819.2 |
| UniProt Secondary Accession: | O95819,O75172, Q9NST7, |
| UniProt Related Accession: | O95819 |
| Molecular Weight: | 1239 |
| NCBI Full Name: | Mitogen-activated protein kinase kinase kinase kinase 4 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase kinase 4 |
| NCBI Official Symbol: | MAP4K4 |
| NCBI Official Synonym Symbols: | HGK; NIK; MEKKK4; FLH21957; HEL-S-31 |
| NCBI Protein Information: | mitogen-activated protein kinase kinase kinase kinase 4; MEK kinase kinase 4; nck-interacting kinase; HPK/GCK-like kinase HGK; Ste20 group protein kinase HGK; MAPK/ERK kinase kinase kinase 4; epididymis secretory protein Li 31; hepatocyte progenitor kinase-like/germinal center kinase-like kinase |
| UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase kinase 4 |
| UniProt Synonym Protein Names: | HPK/GCK-like kinase HGK; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; Nck-interacting kinase |
| Protein Family: | Mitogen-activated protein kinase kinase kinase kinase |
| UniProt Gene Name: | MAP4K4 |
| UniProt Entry Name: | M4K4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAP4K4 antibody (CAB11706) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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