Anti-MAP3K8 Antibody (CAB15623)
- SKU:
- CAB15623
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| 抗体名: | Anti-MAP3K8 Antibody |
| 抗体コード: | CAB15623 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K8 (NP_005195.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP3K8 (NP_005195.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MEYM STGS DNKE EIDL LIKH LNVS DVID IMEN LYAS EEPA VYEP SLMT MCQD SNQN DERS KSLL LSGQ EVPW LSSV RYGT VEDL LAFA NHIS NTAK HFYG |
| 遺伝子ID: | 1326 |
| Uniprot: | P41279 |
| セルラーロケーション: | |
| 計算された分子量: | 49kDa/52kDa |
| 観察された分子量: | 58kDa |
| 同義語: | MAP3K8, AURA2, COT, EST, ESTF, MEKK8, TPL2, Tpl-2, c-COT |
| バックグラウンド: | This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. |
| UniProt Protein Function: | Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.11.25; STE group; STE-Unique family Chromosomal Location of Human Ortholog: 10p11.23 Cellular Component: cytoplasm; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity Biological Process: activation of MAPKK activity; apoptosis; regulation of cell differentiation; T cell costimulation; MAPKKK cascade; cell cycle; protein amino acid phosphorylation Disease: Lung Cancer |
| NCBI Summary: | This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011] |
| UniProt Code: | P41279 |
| NCBI GenInfo Identifier: | 346644795 |
| NCBI Gene ID: | 1326 |
| NCBI Accession: | NP_001231063.1 |
| UniProt Secondary Accession: | P41279,Q14275, Q5T855, Q9HC81, A8K2Q5, D3DRX1, |
| UniProt Related Accession: | P41279 |
| Molecular Weight: | 52,925 Da |
| NCBI Full Name: | mitogen-activated protein kinase kinase kinase 8 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 8 |
| NCBI Official Symbol: | MAP3K8 |
| NCBI Official Synonym Symbols: | COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT |
| NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 8; proto-oncogene c-Cot; tumor progression locus 2; Ewing sarcoma transformant; cot (cancer Osaka thyroid) oncogene; proto-oncogene serine/threoine protein kinase |
| UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 8 |
| UniProt Synonym Protein Names: | Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2 |
| UniProt Gene Name: | MAP3K8 |
| UniProt Entry Name: | M3K8_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAP3K8 antibody (CAB15623) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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