Anti-MAP3K12 Antibody (CAB8464)
- SKU:
- CAB8464
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MAP3K12 Antibody |
| 抗体コード: | CAB8464 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human MAP3K12 (NP_001180440.1). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | SH-SY5Y, HT-1080 |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human MAP3K12 (NP_001180440.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MACL HETR TPSP SFGG FVST LSEA SMRK LDPD TSDC TPEK DLTP TQCV LRDV VPLG GQGG GGPS PSPG GEPP PEPF ANSV LQLH EQDA GGPG GAAG SPES RASR VRAD EVRL QCQS GSGF LEGL FGCL RPVW TMIG KAYS TEHK QQQE DLWE VPFE EILD LQWV GSGA QGAV FLGR FHGE EVAV KKVR DLKE TDIK HLRK LKHP NIIT FK |
| 遺伝子ID: | 7786 |
| Uniprot: | Q12852 |
| セルラーロケーション: | Cytoplasm, Membrane, Peripheral membrane protein |
| 計算された分子量: | 93kDa/96kDa |
| 観察された分子量: | 120kDa |
| 同義語: | MAP3K12, DLK, MEKK12, MUK, ZPK, ZPKP1 |
| バックグラウンド: | This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described. |
| UniProt Protein Function: | DLK: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. |
| UniProt Protein Details: | Protein type:EC 2.7.11.25; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; MLK family; LZK subfamily Chromosomal Location of Human Ortholog: 12q13 Cellular Component: cytoplasm; cytosol; membrane; plasma membrane Molecular Function:protein binding; protein homodimerization activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity Biological Process: histone phosphorylation; JNK cascade; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation |
| NCBI Summary: | This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010] |
| UniProt Code: | Q12852 |
| NCBI GenInfo Identifier: | 116242624 |
| NCBI Gene ID: | 7786 |
| NCBI Accession: | Q12852.2 |
| UniProt Secondary Accession: | Q12852,Q86VQ5, Q8WY25, B3KSS9, G3V1Y2, |
| UniProt Related Accession: | Q12852 |
| Molecular Weight: | 96,322 Da |
| NCBI Full Name: | Mitogen-activated protein kinase kinase kinase 12 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase kinase 12 |
| NCBI Official Symbol: | MAP3K12 |
| NCBI Official Synonym Symbols: | DLK; MUK; ZPK; ZPKP1; MEKK12 |
| NCBI Protein Information: | mitogen-activated protein kinase kinase kinase 12 |
| UniProt Protein Name: | Mitogen-activated protein kinase kinase kinase 12 |
| UniProt Synonym Protein Names: | Dual leucine zipper bearing kinase; DLK; Leucine-zipper protein kinase; ZPK; MAPK-upstream kinase; MUK; Mixed lineage kinase |
| Protein Family: | Mitogen-activated protein kinase kinase kinase |
| UniProt Gene Name: | MAP3K12 |
| UniProt Entry Name: | M3K12_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAP3K12 antibody (CAB8464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
View AllClose