Anti-MAP2K2 Antibody (CAB14770)[KO Validated]
![Anti-MAP2K2 Antibody (CAB14770)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/608x608/products/15741/15625/anti-map2k2-antibody-cab14770ko-validated__14771__77295.1645803083.jpg?c=1 "Anti-MAP2K2 Antibody (CAB14770)[KO Validated]")
- SKU:
- CAB14770
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
![Anti-MAP2K2 Antibody (CAB14770)[KO Validated]](https://cdn11.bigcommerce.com/s-d5atsu6k01/images/stencil/1280x1280/products/15741/15625/anti-map2k2-antibody-cab14770ko-validated__14771__77295.1645803083.jpg?c=1&imbypass=on "Anti-MAP2K2 Antibody (CAB14770)[KO Validated]")
Description
| 抗体名: | Anti-MAP2K2 Antibody [KO Validated] |
| 抗体コード: | CAB14770 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IHC IF |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K2 (NP_109587.1). |
| 申し込み: | WB IHC IF |
| 推奨希釈: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | HeLa |
| 免疫原: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP2K2 (NP_109587.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MLAR RKPV LPAL TINP TIAE GPSP TSEG ASEA NLVD LQKK LEEL ELDE QQKK RLEA FLTQ KAKV GELK DDDF ERIS ELGA GNGG VVTK VQHR PSGL IMAR |
| 遺伝子ID: | 5605 |
| Uniprot: | P36507 |
| セルラーロケーション: | Cytoplasm, Membrane, Peripheral membrane protein |
| 計算された分子量: | 44kDa |
| 観察された分子量: | 50kDa |
| 同義語: | CFC4, MAPKK2, MEK2, MKK2, PRKMK2, MAP2K2 |
| バックグラウンド: | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. |
| UniProt Protein Function: | MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction. |
| UniProt Protein Details: | Protein type:EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; Kinase, protein; STE group; STE7 family Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: Golgi apparatus; peroxisomal membrane; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; extracellular region; intercellular junction; cell cortex; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus Molecular Function:MAP kinase kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activator activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; protein complex scaffold; ATP binding; PDZ domain binding Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of stress-activated MAPK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; toll-like receptor 4 signaling pathway Disease: Cardiofaciocutaneous Syndrome 4 |
| NCBI Summary: | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, cognitive disability, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P36507 |
| NCBI GenInfo Identifier: | 547915 |
| NCBI Gene ID: | 5605 |
| NCBI Accession: | P36507.1 |
| UniProt Related Accession: | P36507 |
| Molecular Weight: | |
| NCBI Full Name: | Dual specificity mitogen-activated protein kinase kinase 2 |
| NCBI Synonym Full Names: | mitogen-activated protein kinase kinase 2 |
| NCBI Official Symbol: | MAP2K2 |
| NCBI Official Synonym Symbols: | CFC4; MEK2; MKK2; MAPKK2; PRKMK2 |
| NCBI Protein Information: | dual specificity mitogen-activated protein kinase kinase 2 |
| UniProt Protein Name: | Dual specificity mitogen-activated protein kinase kinase 2 |
| UniProt Synonym Protein Names: | ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2 |
| UniProt Gene Name: | MAP2K2 |
| UniProt Entry Name: | MP2K2_HUMAN |
![Anti-MAP2K2 Antibody [KO Validated] (CAB14770)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/w/340/A14770_1__69569.jpg) | Western blot analysis of extracts of HeLa cells, using MAP2K2 antibody (CAB14770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
![Anti-MAP2K2 Antibody [KO Validated] (CAB14770)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/w/212/A14770_2__88848.jpg) | Western blot analysis of extracts from normal (control) and MAP2K2 knockout (KO) 293T cells, using MAP2K2 antibody (CAB14770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
![Anti-MAP2K2 Antibody [KO Validated] (CAB14770)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/m/214/A14770_3__04882.jpg) | Immunofluorescence analysis of NIH/3T3 cells using MAP2K2 antibody (CAB14770) at dilution of 1:100. Blue: DAPI for nuclear staining. |
![Anti-MAP2K2 Antibody [KO Validated] (CAB14770)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/o/501/A14770_4__45533.jpg) | Immunofluorescence analysis of U2OS cells using MAP2K2 antibody (CAB14770) at dilution of 1:100. Blue: DAPI for nuclear staining. |