Anti-MANEA Antibody (CAB10756)
- SKU:
- CAB10756
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MANEA Antibody |
| 抗体コード: | CAB10756 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human MANEA (NP_078917.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human |
| ポジティブサンプル: | 293T, U-87MG |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human MANEA (NP_078917.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | RPNT ATFG APFG LDLL PELH QRTI HLGK NFDF QKSD RINS ETNT KNLK SVEI TMKP SKAS ELNL DELP PLN |
| 遺伝子ID: | 79694 |
| Uniprot: | Q5SRI9 |
| セルラーロケーション: | Golgi apparatus membrane, Single-pass type II membrane protein |
| 計算された分子量: | 53kDa |
| 観察された分子量: | 54kDa |
| 同義語: | MANEA, ENDO, hEndo |
| バックグラウンド: |
| UniProt Protein Function: | MANEA: Belongs to the glycosyl hydrolase 99 family |
| UniProt Protein Details: | Protein type:EC 3.2.1.130; Hydrolase; Membrane protein, integral Chromosomal Location of Human Ortholog: 6q16.1 Cellular Component: Golgi apparatus; Golgi membrane Molecular Function:glycoprotein endo-alpha-1,2-mannosidase activity |
| NCBI Summary: | N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008] |
| UniProt Code: | Q5SRI9 |
| NCBI GenInfo Identifier: | 74743637 |
| NCBI Gene ID: | 79694 |
| NCBI Accession: | Q5SRI9.1 |
| UniProt Secondary Accession: | Q5SRI9,Q5SRJ0, Q6MZV0, Q70JE9, Q7Z3V7, Q8WWX5, Q9H9D2 A6H8M6, |
| UniProt Related Accession: | Q5SRI9 |
| Molecular Weight: | 54kDa |
| NCBI Full Name: | Glycoprotein endo-alpha-1,2-mannosidase |
| NCBI Synonym Full Names: | mannosidase endo-alpha |
| NCBI Official Symbol: | MANEA |
| NCBI Official Synonym Symbols: | ENDO; hEndo |
| NCBI Protein Information: | glycoprotein endo-alpha-1,2-mannosidase |
| UniProt Protein Name: | Glycoprotein endo-alpha-1,2-mannosidase |
| UniProt Synonym Protein Names: | Mandaselin |
| Protein Family: | Glycoprotein endo-alpha-1,2-mannosidase |
| UniProt Gene Name: | MANEA |
 | Western blot analysis of extracts of various cell lines, using MANEA antibody (CAB10756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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