Anti-MAN1B1 Antibody (CAB13784)
- SKU:
- CAB13784
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-MAN1B1 Antibody |
| 抗体コード: | CAB13784 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human MAN1B1 (NP_057303.2). |
| 申し込み: | WB |
| 推奨希釈: | WB 1:500 - 1:2000 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | U-87MG, A-549, HeLa, A-431, Mouse kidney, Mouse spleen, Rat brain |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human MAN1B1 (NP_057303.2). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MAAC EGRR SGAL GSSQ SDFL TPPV GGAP WAVA TTVV MYPP PPPP PHRD FISV TLSF GENY DNSK SWRR RSCW RKWK QLSR LQRN MILF LLAF LLFC GLLF YINL ADHW KALA FRLE EEQK MRPE IAGL KPAN PPVL PAPQ KADT DPEN LPEI SSQK TQRH IQRG PPHL QIRP PSQD LKDG TQEE ATKR QEAP VDPR PEGD PQRT VISW RGAV IEPE QGTE LPSR RAEV PTKP PLPP ARTQ GTPV HLNY RQKG VIDV FLHA WKGY RKFA WGHD ELKP VSRS FSEW FGLG LT |
| 遺伝子ID: | 11253 |
| Uniprot: | Q9UKM7 |
| セルラーロケーション: | Endoplasmic reticulum membrane, Single-pass type II membrane protein |
| 計算された分子量: | 79kDa |
| 観察された分子量: | 80kDa |
| 同義語: | MAN1B1, ERMAN1, ERManI, MANA-ER, MRT15 |
| バックグラウンド: | This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1, 2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. |
| UniProt Protein Function: | MAN1B1: Involved in glycoprotein quality control targeting of misfolded glycoproteins for degradation. It primarily trims a single alpha-1,2-linked mannose residue from Man(9)GlcNAc(2) to produce Man(8)GlcNAc(2), but at high enzyme concentrations, as found in the ER quality control compartment (ERQC), it further trims the carbohydrates to Man(5-6)GlcNAc(2). Defects in MAN1B1 are the cause of mental retardation autosomal recessive type 15 (MRT15). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the glycosyl hydrolase 47 family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; EC 3.2.1.113; Hydrolase; Glycan Metabolism - N-glycan biosynthesis; Endoplasmic reticulum; Calcium-binding Chromosomal Location of Human Ortholog: 9q34 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane; vesicle Molecular Function:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; calcium ion binding Biological Process: ER-associated protein catabolic process; cellular protein metabolic process; protein folding; protein amino acid N-linked glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; oligosaccharide metabolic process Disease: Mental Retardation, Autosomal Recessive 15 |
| UniProt Code: | Q9UKM7 |
| NCBI GenInfo Identifier: | 218749883 |
| NCBI Gene ID: | 11253 |
| NCBI Accession: | NP_057303 |
| UniProt Secondary Accession: | Q9UKM7,Q5VSG3, Q9BRS9, Q9Y5K7, |
| UniProt Related Accession: | Q9UKM7 |
| Molecular Weight: | 79,580 Da |
| NCBI Full Name: | endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase |
| NCBI Synonym Full Names: | mannosidase, alpha, class 1B, member 1 |
| NCBI Official Symbol: | MAN1B1 |
| NCBI Official Synonym Symbols: | MRT15; ERMAN1; MANA-ER |
| NCBI Protein Information: | endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase; ER alpha 1,2-mannosidase; Man9GlcNAc2-specific processing alpha-mannosidase; endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1 |
| UniProt Protein Name: | Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase |
| UniProt Synonym Protein Names: | ER alpha-1,2-mannosidase; ER mannosidase 1; ERMan1; Man9GlcNAc2-specific-processing alpha-mannosidase; Mannosidase alpha class 1B member 1 |
| Protein Family: | Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase |
| UniProt Gene Name: | MAN1B1 |
| UniProt Entry Name: | MA1B1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using MAN1B1 antibody (CAB13784) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
View AllClose