Anti-LMNB1 Antibody (CAB16685)
- SKU:
- CAB16685
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-LMNB1 Antibody |
| 抗体コード: | CAB16685 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF IP |
| 反応性: | Human, Mouse, Rat |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant protein of human LMNB1 |
| 申し込み: | WB IF IP |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| 反応性: | Human, Mouse, Rat |
| ポジティブサンプル: | Jurkat, MCF7, mouse brain, mouse spleen |
| 免疫原: | Recombinant protein of human LMNB1 |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | Email for sequence |
| 遺伝子ID: | 4001 |
| Uniprot: | P20700 |
| セルラーロケーション: | Lipid-anchor, Nucleoplasmic side, Nucleus inner membrane |
| 計算された分子量: | 66kDa |
| 観察された分子量: | 70kDa |
| 同義語: | ADLD, LMN, LMN2, LMNB, Lamin B1, LMNB1, lamin-B1 |
| バックグラウンド: | This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 5q23.2 Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope Molecular Function:phospholipase binding; structural molecule activity Biological Process: apoptosis; cell structure disassembly during apoptosis Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant |
| NCBI Summary: | This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P20700 |
| NCBI GenInfo Identifier: | 125953 |
| NCBI Gene ID: | 4001 |
| NCBI Accession: | P20700.2 |
| UniProt Related Accession: | P20700 |
| Molecular Weight: | ~ 68kDa |
| NCBI Full Name: | Lamin-B1 |
| NCBI Synonym Full Names: | lamin B1 |
| NCBI Official Symbol: | LMNB1 |
| NCBI Official Synonym Symbols: | LMN; ADLD; LMN2; LMNB |
| NCBI Protein Information: | lamin-B1 |
| UniProt Protein Name: | Lamin-B1 |
| Protein Family: | Light-harvesting protein |
| UniProt Gene Name: | LMNB1 |
| UniProt Entry Name: | LMNB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (CAB16685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Confocal immunofluorescence analysis of HeLa cells using LMNB1 antibody (CAB16685) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining. |
 | Confocal immunofluorescence analysis of MCF-7 cells using LMNB1 antibody (CAB16685) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining. |
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