Anti-LIMK2 Antibody (CAB5409)
- SKU:
- CAB5409
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| 抗体名: | Anti-LIMK2 Antibody |
| 抗体コード: | CAB5409 |
| 抗体サイズ: | 20uL, 50uL, 100uL |
| 申し込み: | WB IF |
| 反応性: | Human, Mouse |
| 宿主種: | Rabbit |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LIMK2 (NP_001026971.1). |
| 申し込み: | WB IF |
| 推奨希釈: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| 反応性: | Human, Mouse |
| ポジティブサンプル: | HepG2, HeLa, Mouse pancreas |
| 免疫原: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human LIMK2 (NP_001026971.1). |
| 精製方法: | Affinity purification |
| ストレージバッファ: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| アイソタイプ: | IgG |
| 順序: | MGSY LSVP AYFT SRDL FRCS ECQD SLTN WYYE KDGK LYCP KDYW GKFG EFCH GCSL LMTG PFMV AGEF KYHP ECFA CMSC KVII EDGD AYAL VQHA TLYC GKCH NEVV LAPM FERL STES VQEQ LPYS VTLI SMPA TTEG RRGF SVSV ESAC SNYA TTVQ VKEV NRMH ISPN NRNA IHPG DRIL EING TPVR TLRV EEVE DAIS QTSQ TLQL LIEH DPVS QRLD QLRL EARL APHM QNAG |
| 遺伝子ID: | 3985 |
| Uniprot: | P53671 |
| セルラーロケーション: | Cytoplasm, Nucleus, Nucleus |
| 計算された分子量: | 69kDa/72kDa/77kDa |
| 観察された分子量: | 80kDa |
| 同義語: | LIMK2 |
| バックグラウンド: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | LIMK2: a TKL kinase of the LISK family that regulates actin dynamics. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. Phosphorylates cofilin, inhibiting its actin-depolymerizing activity. May contributes to Rho-induced reorganization of the actin cytoskeleton. Two alternative splice variants have been identified. |
| UniProt Protein Details: | Protein type:Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; TKL group; LISK family; LIMK subfamily Chromosomal Location of Human Ortholog: 22q12.2 Cellular Component: cis-Golgi network; cytoplasm; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; ATP binding Biological Process: spermatogenesis; phosphorylation; protein amino acid phosphorylation |
| NCBI Summary: | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P53671 |
| NCBI GenInfo Identifier: | 1708824 |
| NCBI Gene ID: | 3985 |
| NCBI Accession: | P53671.1 |
| UniProt Secondary Accession: | P53671,Q7KZ80, Q7L3H5, Q96E10, Q99464, Q9UFU0, A8K6H5 |
| UniProt Related Accession: | P53671 |
| Molecular Weight: | 638 |
| NCBI Full Name: | LIM domain kinase 2 |
| NCBI Synonym Full Names: | LIM domain kinase 2 |
| NCBI Official Symbol: | LIMK2 |
| NCBI Protein Information: | LIM domain kinase 2 |
| UniProt Protein Name: | LIM domain kinase 2 |
| Protein Family: | LIM domain kinase |
| UniProt Gene Name: | LIMK2 |
| UniProt Entry Name: | LIMK2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LIMK2 antibody (CAB5409) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of L929 cells using LIMK2 Rabbit pAb (CAB5409) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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